Lipoxygenase activity in ontogenesis of ferns Salvinia natans and Polystichum aculeatum

Abstract

We analyzed the peculiarities of lipoxygenase (LOX) localization and activity in organs of two higher vascular cryptogams at the different phenological phases of development. For the first time it was showed that floating fronds of the annual water fern Salvinia natans (L.) contained 13-LOX with рНopt value of 8.0, when submerged ones – 13-LOX (рНopt 8.0) at the initial phenological phases of development and 9-LOX (рНopt 5.5) at the subsequent phases. In sporocarps 9-LOX (рНоpt 5.5) was identified. At the same time in fronds of the perennial land fern Polystichum aculeatum (L.) Roth. we identified 13-LOX with рНopt value of 7.74, and in the rhizome – 9-LOX (рНopt 7.54). The localization pattern and change of LOXs isoform activity in ferns organs in various phenological phases of development allow us to suggest that enzyme may be involved in the regulation of growth processes that ensure adaptation to environmental conditions.

Keywords

• lipoxigenases
• Polystichum aculeatum (L.) Roth.
• Salvinia natans (L.) All.