Nature Communications (Apr 2020)
Stem cell-derived polarized hepatocytes
- Viet Loan Dao Thi,
- Xianfang Wu,
- Rachel L. Belote,
- Ursula Andreo,
- Constantin N. Takacs,
- Joseph P. Fernandez,
- Luis Andre Vale-Silva,
- Sarah Prallet,
- Charlotte C. Decker,
- Rebecca M. Fu,
- Bingqian Qu,
- Kunihiro Uryu,
- Henrik Molina,
- Mohsan Saeed,
- Eike Steinmann,
- Stephan Urban,
- Roshni R. Singaraja,
- William M. Schneider,
- Sanford M. Simon,
- Charles M. Rice
Affiliations
- Viet Loan Dao Thi
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- Xianfang Wu
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- Rachel L. Belote
- Laboratory of Cellular Biophysics, The Rockefeller University
- Ursula Andreo
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- Constantin N. Takacs
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- Joseph P. Fernandez
- Proteomics Resource Center, The Rockefeller University
- Luis Andre Vale-Silva
- Department of Biology, New York University
- Sarah Prallet
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- Charlotte C. Decker
- Schaller Research Group at Department of Infectious Diseases and Virology, Heidelberg University Hospital, Cluster of Excellence CellNetworks
- Rebecca M. Fu
- Schaller Research Group at Department of Infectious Diseases and Virology, Heidelberg University Hospital, Cluster of Excellence CellNetworks
- Bingqian Qu
- Department of Infectious Diseases and Virology, Heidelberg University Hospital, Cluster of Excellence CellNetworks
- Kunihiro Uryu
- Electron Microscopy Resource Center, The Rockefeller University
- Henrik Molina
- Proteomics Resource Center, The Rockefeller University
- Mohsan Saeed
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- Eike Steinmann
- Department of Molecular and Medical Virology, Ruhr-University Bochum
- Stephan Urban
- Department of Infectious Diseases and Virology, Heidelberg University Hospital, Cluster of Excellence CellNetworks
- Roshni R. Singaraja
- A*STAR (Agency for Science, Technology and Research) Institute and Yong Loo Lin School of Medicine, National University of Singapore
- William M. Schneider
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- Sanford M. Simon
- Laboratory of Cellular Biophysics, The Rockefeller University
- Charles M. Rice
- Laboratory of Virology and Infectious Diseases, The Rockefeller University
- DOI
- https://doi.org/10.1038/s41467-020-15337-2
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
To model hepatocyte function accurately in vitro, it is necessary to generate and maintain a polarized epithelium. Here, the authors describe a protocol to generate polarized human pluripotent stem cell-derived hepatocyte-like cells (HLCs) to model enteric virus production and drug secretion in vitro.
