RNF11 negatively regulates antiviral signaling and incites inflammatory response in chicken embryo fibroblast

Abstract

RNF11 (RING finger protein 11) has been indicated to be related to numerous immunological diseases. As a neo-regulator in innate immunity, RNF11 was proved to inhibit antiviral signaling and interferon-β (IFN-β) production in human cell line. Chicken antiviral signaling pathway is different with other species. However, there is little study about the role of RNF11 in chicken antiviral signaling pathway and inflammatory response. This study was designed to identify the function of RNF11 in chicken antiviral signaling pathway and inflammatory response. We overexpressed and interfered RNF11 in chicken embryo fibroblasts (CEFs) and then used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to investigate the poly (I:C)-induced production of IFN-β and inflammatory response factors IL2 and IL10 . The results indicated that RNF11 extremely significantly decreased the production of IFN-β and highly significantly increased the messenger RNA (mRNA) levels of IL2 and IL10 , but depletion of RNF11 obtained inverse results. Thus, we concluded that RNF11 not only can inhibit antiviral signaling but also plays an important role in inflammatory response in chicken .