Electrospinning is often investigated for biotechnological applications, such as tissue engineering and cell growth in general. In many cases, three-dimensional scaffolds would be advantageous to prepare tissues in a desired shape. Some studies thus investigated 3D-printed scaffolds decorated with electrospun nanofibers. Here, we report on the influence of 3D-printed substrates on fiber orientation and diameter of a nanofiber mat, directly electrospun on conductive and isolating 3D-printed objects, and show the effect of shadowing, taking 3D-printed ears with electrospun nanofiber mats as an example for potential and direct application in tissue engineering in general.