Frontiers in Bioengineering and Biotechnology (Feb 2020)

Review on D-Allulose: In vivo Metabolism, Catalytic Mechanism, Engineering Strain Construction, Bio-Production Technology

  • Suwei Jiang,
  • Suwei Jiang,
  • Wei Xiao,
  • Xingxing Zhu,
  • Peizhou Yang,
  • Zhi Zheng,
  • Shuhua Lu,
  • Shaotong Jiang,
  • Guochang Zhang,
  • Jingjing Liu

DOI
https://doi.org/10.3389/fbioe.2020.00026
Journal volume & issue
Vol. 8

Abstract

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Rare sugar D-allulose as a substitute sweetener is produced through the isomerization of D-fructose by D-tagatose 3-epimerases (DTEases) or D-allulose 3-epimerases (DAEases). D-Allulose is a kind of low energy monosaccharide sugar naturally existing in some fruits in very small quantities. D-Allulose not only possesses high value as a food ingredient and dietary supplement, but also exhibits a variety of physiological functions serving as improving insulin resistance, antioxidant enhancement, and hypoglycemic controls, and so forth. Thus, D-allulose has an important development value as an alternative to high-energy sugars. This review provided a systematic analysis of D-allulose characters, application, enzymatic characteristics and molecular modification, engineered strain construction, and processing technologies. The existing problems and its proposed solutions for D-allulose production are also discussed. More importantly, a green and recycling process technology for D-allulose production is proposed for low waste formation, low energy consumption, and high sugar yield.

Keywords