Practical tips and common mistakes in ocular microbiology sampling and processing

Abstract

Ocular microbiology deals with miniscule samples from ocular infections, which are difficult to collect, process, and analyze, necessitating special skills, and the knowledge of troubleshooting errors to reach a specific diagnosis. In this article, we highlight several practical points in ocular microbiology, common mistakes, and various approaches to resolve them. We have covered sample collection from different ocular compartments, processing for smear preparation and culture, transport of samples, staining and reagents issues, artifacts and contaminants, and interpretation of in-vitro antimicrobial susceptibility testing reports. This review aims to help both ophthalmologists and microbiologists in making the practice of ocular microbiology and the interpretation of reports more reliable, hassle-free, and precise.

Keywords

• antimicrobial susceptibility testing
• dye contamination
• microbiology artifacts
• microbiology sampling errors
• ocular microbiology
• sampling nucleic acid amplification test