Spectrophotometric Determination of Fe(II) and Fe(III) Using 5-(4-Ethylphenyl azo ) 2-Hydroxybenzaldehyde Oxime Reagent

Abstract

Simple, rapid and sensitive spectrophotometric methods for the determination of trace amounts of iron (II) and iron (III) were developed based on complex formation between 5-(4- Ethylphenyl azo )2-Hydroxybenzaldehyde oxime reagent with iron (II) and iron (III) in acidic medium. The reagent 5-(4-Ethylphenyl azo )2-Hydroxybenzaldehyde oxime has been synthesized and used for iron analysis. The calibration graphs were linear in the concentration range 1.0-6.0 µg/ml and1.0-7.0 µg/ml of iron (II) and iron (III), respectively. The total iron also was determined after reduction of iron (III) to iron (II) employing ascorbic acid solution. The effects of foreign ions and species on the determination of Fe (II) and Fe (III) were investigated to assess the selectivity of the methods. The proposed methods were successfully applied for iron determination in pure form and in pharmaceutical preparation. Good agreement was obtained between the results of the proposed methods and by the reference method.

Keywords

• iron
• spectrophotometry
• azo-oxime ligand