Genetic Renal Diseases: The Emerging Role of Zebrafish Models
Mohamed A. Elmonem,
Sante Princiero Berlingerio,
Lambertus P. van den Heuvel,
Peter A. de Witte,
Martin Lowe,
Elena N. Levtchenko
Affiliations
Mohamed A. Elmonem
Department of Pediatric Nephrology & Development and Regeneration, University Hospitals Leuven, KU Leuven—University of Leuven, Herestraat 49, Box 817, 3000 Leuven, Belgium
Sante Princiero Berlingerio
Department of Pediatric Nephrology & Development and Regeneration, University Hospitals Leuven, KU Leuven—University of Leuven, Herestraat 49, Box 817, 3000 Leuven, Belgium
Lambertus P. van den Heuvel
Department of Pediatric Nephrology & Development and Regeneration, University Hospitals Leuven, KU Leuven—University of Leuven, Herestraat 49, Box 817, 3000 Leuven, Belgium
Peter A. de Witte
Laboratory for Molecular Bio-Discovery, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven—University of Leuven, 3000 Leuven, Belgium
Martin Lowe
Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PL, UK
Elena N. Levtchenko
Department of Pediatric Nephrology & Development and Regeneration, University Hospitals Leuven, KU Leuven—University of Leuven, Herestraat 49, Box 817, 3000 Leuven, Belgium
The structural and functional similarity of the larval zebrafish pronephros to the human nephron, together with the recent development of easier and more precise techniques to manipulate the zebrafish genome have motivated many researchers to model human renal diseases in the zebrafish. Over the last few years, great advances have been made, not only in the modeling techniques of genetic diseases in the zebrafish, but also in how to validate and exploit these models, crossing the bridge towards more informative explanations of disease pathophysiology and better designed therapeutic interventions in a cost-effective in vivo system. Here, we review the significant progress in these areas giving special attention to the renal phenotype evaluation techniques. We further discuss the future applications of such models, particularly their role in revealing new genetic diseases of the kidney and their potential use in personalized medicine.
