Characterization of Inulin-Type Fructan from <i>Platycodon grandiflorus</i> and Study on Its Prebiotic and Immunomodulating Activity
De-Jiang Pang,
Chao Huang,
Mei-Ling Chen,
Yu-Long Chen,
Yu-Ping Fu,
Berit Smestad Paulsen,
Frode Rise,
Bing-Zhao Zhang,
Zheng-Li Chen,
Ren-Yong Jia,
Li-Xia Li,
Xu Song,
Bin Feng,
Xue-Qin Ni,
Zhong-Qiong Yin,
Yuan-Feng Zou
Affiliations
De-Jiang Pang
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Chao Huang
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Mei-Ling Chen
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yu-Long Chen
Sichuan Academy of Forestry, Chengdu 610081, China
Yu-Ping Fu
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Berit Smestad Paulsen
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway
Frode Rise
Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway
Bing-Zhao Zhang
Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China
Zheng-Li Chen
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Ren-Yong Jia
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Li-Xia Li
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Xu Song
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Bin Feng
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China
Xue-Qin Ni
Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Zhong-Qiong Yin
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yuan-Feng Zou
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Platycodon grandiflorus is a plant widely used in traditional Chinese medicine, of which polysaccharides are reported to be the main components responsible for its bio-functions. In this work, the inulin-type fructan (PGF) was obtained by DEAE anion exchange chromatography from the water extracted from P. grandifloras. Characterization was performed with methanolysis, methylation, and NMR and the results showed that PGF is a β-(2-1) linked fructan, with terminal glucose and with a degree of polymerization of 2–10. In order to study its biofunctions, the prebiotic and immunomodulation properties were assayed. We found that PGF exhibited good prebiotic activity, as shown by a promotion on six strains of lactobacillus proliferation. Additionally, the PGF also displayed direct immunomodulation on intestinal epithelial cells and stimulated the expressions of anti-inflammatory factors. These results indicated that the inulin from P. grandiflorus is a potential natural source of prebiotics as well as a potential intestinal immunomodulator, which will be valuable for further studies and new applications.
