Frontiers in Public Health (Oct 2022)
An improvement of current hypercube pooling PCR tests for SARS-CoV-2 detection
- Tai-Yin Wu,
- Tai-Yin Wu,
- Tai-Yin Wu,
- Yu-Ciao Liao,
- Chiou-Shann Fuh,
- Pei-Wei Weng,
- Pei-Wei Weng,
- Pei-Wei Weng,
- Jr-Yi Wang,
- Jr-Yi Wang,
- Chih-Yu Chen,
- Chih-Yu Chen,
- Chih-Yu Chen,
- Chih-Yu Chen,
- Yu-Min Huang,
- Yu-Min Huang,
- Chung-Pei Chen,
- Yo-Lun Chu,
- Yo-Lun Chu,
- Yo-Lun Chu,
- Cheng-Kuang Chen,
- Cheng-Kuang Chen,
- Kuei-Lin Yeh,
- Kuei-Lin Yeh,
- Kuei-Lin Yeh,
- Ching-Hsiao Yu,
- Ching-Hsiao Yu,
- Hung-Kang Wu,
- Hung-Kang Wu,
- Hung-Kang Wu,
- Wei-Peng Lin,
- Wei-Peng Lin,
- Tsan-Hon Liou,
- Mai-Szu Wu,
- Chen-Kun Liaw,
- Chen-Kun Liaw,
- Chen-Kun Liaw,
- Chen-Kun Liaw
Affiliations
- Tai-Yin Wu
- Department of Family Medicine, Zhongxing Branch, Taipei City Hospital, Taipei, Taiwan
- Tai-Yin Wu
- Institute of Epidemiology and Preventive Medicine, National Taiwan University, Taipei, Taiwan
- Tai-Yin Wu
- General Education Center, University of Taipei, Taipei, Taiwan
- Yu-Ciao Liao
- Institute of Computer Science and Information Engineering, National Taiwan University, Taipei, Taiwan
- Chiou-Shann Fuh
- Institute of Computer Science and Information Engineering, National Taiwan University, Taipei, Taiwan
- Pei-Wei Weng
- Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
- Pei-Wei Weng
- Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei, Taiwan
- Pei-Wei Weng
- Graduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Research Center of Biomedical Device, Taipei Medical University, Taipei, Taiwan
- Jr-Yi Wang
- Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
- Jr-Yi Wang
- Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei, Taiwan
- Chih-Yu Chen
- Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
- Chih-Yu Chen
- Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei, Taiwan
- Chih-Yu Chen
- Graduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Research Center of Biomedical Device, Taipei Medical University, Taipei, Taiwan
- Chih-Yu Chen
- International Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan
- Yu-Min Huang
- Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
- Yu-Min Huang
- Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei, Taiwan
- Chung-Pei Chen
- Department of Orthopedics, Cathay General Hospital, Taipei, Taiwan
- Yo-Lun Chu
- 0Department of Orthopedics, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
- Yo-Lun Chu
- 1School of Medicine, College of Medicine, Fu Jen Catholic University, Taipei, Taiwan
- Yo-Lun Chu
- 2Department of Biomedical Engineering, National Taiwan University, Taipei, Taiwan
- Cheng-Kuang Chen
- 0Department of Orthopedics, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
- Cheng-Kuang Chen
- 2Department of Biomedical Engineering, National Taiwan University, Taipei, Taiwan
- Kuei-Lin Yeh
- Institute of Computer Science and Information Engineering, National Taiwan University, Taipei, Taiwan
- Kuei-Lin Yeh
- 3Department of Orthopaedics, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi, Taiwan
- Kuei-Lin Yeh
- 4Department of Long-Term Care and Management, WuFeng University, Chiayi, Taiwan
- Ching-Hsiao Yu
- 5Department of Orthopaedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan
- Ching-Hsiao Yu
- 6Department of Orthopaedic Surgery, National Taiwan University Hospital, Taipei, Taiwan
- Hung-Kang Wu
- 5Department of Orthopaedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan
- Hung-Kang Wu
- 6Department of Orthopaedic Surgery, National Taiwan University Hospital, Taipei, Taiwan
- Hung-Kang Wu
- 7Department of Nursing, Yuanpei University of Medical Technology, Hsinchu, Taiwan
- Wei-Peng Lin
- 6Department of Orthopaedic Surgery, National Taiwan University Hospital, Taipei, Taiwan
- Wei-Peng Lin
- 8Department of Orthopedics, Postal Hospital, Taipei, Taiwan
- Tsan-Hon Liou
- 9Department of Physical Medicine and Rehabilitation, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
- Mai-Szu Wu
- 0Division of Nephrology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
- Chen-Kun Liaw
- Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
- Chen-Kun Liaw
- Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei, Taiwan
- Chen-Kun Liaw
- Graduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Research Center of Biomedical Device, Taipei Medical University, Taipei, Taiwan
- Chen-Kun Liaw
- 1TMU Biodesign Center, Taipei Medical University, Taipei, Taiwan
- DOI
- https://doi.org/10.3389/fpubh.2022.994712
- Journal volume & issue
-
Vol. 10
Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic can be effectively controlled by rapid and accurate identification of SARS-CoV-2-infected cases through large-scale screening. Hypercube pooling polymerase chain reaction (PCR) is frequently used as a pooling technique because of its high speed and efficiency. We attempted to implement the hypercube pooling strategy and found it had a large quantization effect. This raised two questions: is hypercube pooling with edge = 3 actually the optimal strategy? If not, what is the best edge and dimension? We used a C++ program to calculate the expected number of PCR tests per patient for different values of prevalence, edge, and dimension. The results showed that every edge had a best performance range. Then, using C++ again, we created a program to calculate the optimal edge and dimension required for pooling samples when entering prevalence into our program. Our program will be provided as freeware in the hope that it can help governments fight the SARS-CoV-2 pandemic.
Keywords
