Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs

Yan Lu; Yan Lu; Yi Xiao; Yi Xiao; Ming-Zhu Yin; Ming-Zhu Yin; Xing-Chen Zhou; Li-Sha Wu; Li-Sha Wu; Wang-Qing Chen; Yan Luo; Yan Luo; Ye-Hong Kuang; Ye-Hong Kuang; Wu Zhu; Wu Zhu

doi:10.3389/fmed.2020.560579

Frontiers in Medicine (Mar 2021)

Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs

Yan Lu,
Yan Lu,
Yi Xiao,
Yi Xiao,
Ming-Zhu Yin,
Ming-Zhu Yin,
Xing-Chen Zhou,
Li-Sha Wu,
Li-Sha Wu,
Wang-Qing Chen,
Yan Luo,
Yan Luo,
Ye-Hong Kuang,
Ye-Hong Kuang,
Wu Zhu,
Wu Zhu

Affiliations

Yan Lu: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Yan Lu: Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China
Yi Xiao: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Yi Xiao: Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China
Ming-Zhu Yin: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Ming-Zhu Yin: Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China
Xing-Chen Zhou: Pharmacy, Xiangya Hospital, Central South University, Changsha, China
Li-Sha Wu: Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China
Li-Sha Wu: Institute of Medical Science, Xiangya Hospital, Central South University, Changsha, China
Wang-Qing Chen: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Yan Luo: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Yan Luo: Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China
Ye-Hong Kuang: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Ye-Hong Kuang: Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China
Wu Zhu: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Wu Zhu: Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China

DOI: https://doi.org/10.3389/fmed.2020.560579
Journal volume & issue: Vol. 7

Abstract

Read online

Objective: To explore the possible mechanism of improving the imiquimod (IMQ)-induced psoriasis-like inflammation by using polyethylene glycol (PEG) ointment.Methods: We evaluated the appearance of psoriasis lesions by Psoriasis Area and Severity Index (PASI), observed the epidermal proliferation by histopathological staining and immunohistochemical staining, and explored the key molecules and signaling pathways of improving psoriasis-like inflammation treated with PEG ointment by RNA sequencing. Finally, we verified the expression of inflammatory cells and inflammatory factors by flow cytometry, immunohistochemical staining, and Q-PCR.Results: PEG ointment could improve the appearance of psoriasis lesions and the epidermis thickness of psoriasis mouse, inhibit the proliferation of keratinocytes, and down-regulate the relative mRNA levels of IL-23, IL-22, IL-6, IL-17C, IL-17F, S100A7, S100A8, S100A9, CXCL1, CXCL2, and IL-1β in the skin lesions of psoriasis mouse by down-regulating the numbers of myeloid-derived suppressor cells (MDSCs) and T helper 17 (Th17) cells.Conclusion: PEG ointment could improve the IMQ-induced psoriasis-like inflammation by down-regulating the functions of Th17 cells and MDSCs.

Published in Frontiers in Medicine

ISSN: 2296-858X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Medicine (General)
Website: http://www.frontiersin.org/journals/medicine

About the journal

Abstract

Keywords