Applied Sciences (Jan 2025)

Validating Accelerated Shelf Life Testing Methodology for Predicting Shelf Life in High-Pressure-Processed Meat Products

  • Athina Ntzimani,
  • Maria Tsevdou,
  • Evangelos Andrianos,
  • Dimitrios Gounaris,
  • Theodosios Spiliotopoulos,
  • Petros Taoukis,
  • Maria C. Giannakourou

DOI
https://doi.org/10.3390/app15031264
Journal volume & issue
Vol. 15, no. 3
p. 1264

Abstract

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The shelf life of meat products is a critical factor in ensuring both consumer safety and product quality. Traditional methods for determining shelf life are labor-intensive and time-consuming, making it challenging for manufacturers to adapt to market demands. The accelerated shelf life testing (ASLT) methodology offers a viable solution by exposing products to controlled elevated conditions that simulate long-term storage, allowing for faster shelf life predictions. This study evaluates the ASLT methodology as a predictive tool for determining the shelf life of high-pressure (HPP)-treated meat products. The present study includes experiments to verify the shelf life of frankfurter-type sausages under accelerated conditions. By simulating long-term storage at elevated temperatures (4–18 °C), a kinetic model was developed to monitor spoilage bacteria growth, with validation under dynamic temperature conditions. The results indicate that the main spoilage population of frankfurter-type sausages was lactic acid bacteria (LAB), which was strongly correlated with the total mesophilic microflora of the products. The findings show that elevated storage temperatures (8 and 18 °C) provide accurate shelf life predictions, offering an efficient approach to ensure product quality and meet consumer demands.

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