Антибиотики и Химиотерапия (May 2020)

Genotypes and β-Lactamase Gene Carriage in Carbapenem-Resistant Acinetobacter Baumannii Isolated in Moscow

  • I. V. Chebotar,
  • O. A. Kryzhanovskaya,
  • N. M. Alyabieva,
  • T. A. Savinova,
  • Yu. A. Bocharova,
  • A. V. Lazareva,
  • S. V. Polikarpova,
  • O. V. Karaseva,
  • N. F. Mayanskiy

Journal volume & issue
Vol. 62, no. 11-12
pp. 29 – 34

Abstract

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The aim of the study was to determine the mechanisms of resistance to carbapenems in Acinetobacter baumannii strains and give their clonal characteristics by multilocus sequence typing. Materia/s and methods. The authors characterized carbapenem-resistant A.baumannii isolates (CRAB) collected from patients in surgical departments and intensive care units at three hospitals in Moscow in 2012-2015. Sensitivity to carbapenems was determined by the Etest method. Identification of sequence types (ST) was carried out by multilocus sequence typing (MLST) using standard Oxford and Pasteur protocols. Detection of β-lactamase genes was performed by PCR. Resu/ts. Among 88 examined CRAB isolates, the Oxford (Oxf) MLST protocol identified 15STs of 3 clonal complexes (CCs), including CC92Oxf (69%), CC109Oxf (1%), CC944Oxf (30%). CC944Oxf was composed of ST944Oxf (n=16) and two newly described single locus variants ST1103Oxf (n=3) and ST1104Oxf (n=7); all three STs were identical to the Pasteur (Pas) MLST protocol ST78. All CC944Oxf/ST78Pas isolates were b/aOXA-72-positive and all but one of them had a b/aCTX_M_115 gene. ST944Oxf was the only ST found in each of the three study hospitals. Conc/usion. Our data demonstrate the predominance of two clonal lineages among Carba-NS A.baumannii. One of them, the uncommon b/aOXA_72/b/aCTX_M_115-positive clone of CC944Oxf/ST78Pas, seems to be endemic in Russia.

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