Toxicology Reports (Jan 2021)
Analysis of the biochemical and histopathological impact of a mild dose of commercial malathion on Channa punctatus (Bloch) fish
Abstract
The intensive application of pesticides without proper disposal management has led their excess residues to reach the neighbouring aquatic ecosystem and its inhabitants mainly fish. In natural water body pesticides get diluted, and therefore to study the silent toxic effect, a low dose of malathion (0.4 mg/L; 1/20th of 96-h LC50 value) for the different duration (1, 4, 8, 12 days) was evaluated through biochemical and histopathological biomarkers of the blood and hepatorenal tissues of Channa punctatus. With the increase in pesticide exposure periods, the biometric indices: Condition Factor (K), HSI and KSI and hepatorenal tissues weight decreased. Among the biochemical alterations in malathion exposed fish, serum glucose levels reduced by 72.23 % while protein amounts increased by 29.03 % in 12 days malathion exposed fish. Other parameters, viz., cholesterol, albumin, and phosphorous, remained the same as control fish after malathion exposure. Though serum bilirubin (total and direct) followed a biphasic response, it reduced by 60 % after 12 days of malathion exposure compared to control. Biochemical changes are reflecting the induction of compensatory energy mechanism to cope up with the malathion stress. The transaminases and ALP biomarker enzymes used for liver functionality test declined in the order of AST > ALP > ALT in a time-dependent manner in malathion exposed fish serum, indicating liver injuries in fish due to malathion. The elevated levels of urea, BUN, creatinine, and Ca2+ in the serum of 12 days of malathion exposed fish revealed renal dysfunction. In the treated fish, antioxidative (SOD and CAT) and LPO activities were significantly higher in the liver followed by the kidney than their controls. Further, histological examination registered progressive damages in the hepatorenal tissues of malathion exposed fish with the increased exposure periods compared to control. Thus, even a small dose of malathion in water could severely deteriorate the structure and function of tissue on its prolonged exposure, and therefore utmost care should be taken to prevent their seepage into the water bodies.
