Nature Communications (Mar 2020)

An expanded library of orthogonal split inteins enables modular multi-peptide assemblies

  • Filipe Pinto,
  • Ella Lucille Thornton,
  • Baojun Wang

DOI
https://doi.org/10.1038/s41467-020-15272-2
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 15

Abstract

Read online

Inteins allow the joining of protein segments through scarless ligation. Here the authors assess 34 inteins to establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which are also active in vitro.