Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria

Aude Bernheim; Alicia Calvo-Villamañán; Clovis Basier; Lun Cui; Eduardo P. C. Rocha; Marie Touchon; David Bikard

doi:10.1038/s41467-017-02350-1

Nature Communications (Dec 2017)

Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria

Aude Bernheim,
Alicia Calvo-Villamañán,
Clovis Basier,
Lun Cui,
Eduardo P. C. Rocha,
Marie Touchon,
David Bikard

Affiliations

Aude Bernheim: Microbial Evolutionary Genomics, Institut Pasteur
Alicia Calvo-Villamañán: Synthetic Biology Group, Institut Pasteur
Clovis Basier: Synthetic Biology Group, Institut Pasteur
Lun Cui: Synthetic Biology Group, Institut Pasteur
Eduardo P. C. Rocha: Microbial Evolutionary Genomics, Institut Pasteur
Marie Touchon: Microbial Evolutionary Genomics, Institut Pasteur
David Bikard: Synthetic Biology Group, Institut Pasteur

DOI: https://doi.org/10.1038/s41467-017-02350-1
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

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The double-strand breaks generated by CRISPR-Cas systems are the target of multiple DNA repair pathways. Here the authors find incompatibility between NHEJ and type II-A CRISPR-Cas systems due to Csn2 mediated inhibition of end-joining.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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