Co-Inoculation of Rhizobacteria and Biochar Application Improves Growth and Nutrientsin Soybean and Enriches Soil Nutrients and Enzymes
Dilfuza Jabborova,
Stephan Wirth,
Annapurna Kannepalli,
Abdujalil Narimanov,
Said Desouky,
Kakhramon Davranov,
R. Z. Sayyed,
Hesham El Enshasy,
Roslinda Abd Malek,
Asad Syed,
Ali H. Bahkali
Affiliations
Dilfuza Jabborova
Laboratory of Medicinal Plants Genetics and Biotechnology, Institute of Genetics and Plant Experimental Biology, Uzbekistan Academy of Sciences, Tashkent Region, Kibray 111208, Uzbekistan
Stephan Wirth
Leibniz Centre for Agricultural Landscape Research (ZALF), D-15374 Müncheberg, Germany
Annapurna Kannepalli
Division of Microbiology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi 110012, India
Abdujalil Narimanov
Laboratory of Medicinal Plants Genetics and Biotechnology, Institute of Genetics and Plant Experimental Biology, Uzbekistan Academy of Sciences, Tashkent Region, Kibray 111208, Uzbekistan
Said Desouky
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11651, Egypt
Kakhramon Davranov
Institute of Microbiology, Academy of Sciences of Uzbekistan, Tashkent 100128, Uzbekistan
R. Z. Sayyed
Department of Microbiology, PSGVP Mandal’s, Arts, Science & Commerce College, Shahada 425409, Maharashtra, India
Hesham El Enshasy
Institute of Bioproduct Development (IBD), Universiti Teknologi, Malaysia (UTM), Skudai, Johor Bahru 81310, Malaysia
Roslinda Abd Malek
Institute of Bioproduct Development (IBD), Universiti Teknologi, Malaysia (UTM), Skudai, Johor Bahru 81310, Malaysia
Asad Syed
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Ali H. Bahkali
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Gradual depletion in soil nutrients has affected soil fertility, soil nutrients, and the activities of soil enzymes. The applications of multifarious rhizobacteria can help to overcome these issues, however, the effect of co-inoculation of plant-growth promoting rhizobacteria (PGPR) and biochar on growth andnutrient levelsin soybean and on the level of soil nutrients and enzymes needs in-depth study. The present study aimed to evaluate the effect of co-inoculation of multifarious Bradyrhizobium japonicum USDA 110 and Pseudomonas putida TSAU1 and different levels (1 and 3%) of biochar on growth parameters and nutrient levelsin soybean and on the level of soil nutrients and enzymes. Effect of co-inoculation of rhizobacteria and biochar (1 and 3%) on the plant growth parameters and soil biochemicals were studied in pot assay experiments under greenhouse conditions. Both produced good amounts of indole-acetic acid; (22 and 16 µg mL−1), siderophores (79 and 87%SU), and phosphate solubilization (0.89 and 1.02 99 g mL−1). Co-inoculation of B. japonicum with P. putida and 3% biochar significantly improved the growth and nutrient content ofsoybean and the level of nutrients and enzymes in the soil, thus making the soil more fertile to support crop yield. The results of this research provide the basis of sustainable and chemical-free farming for improved yields and nutrients in soybean and improvement in soil biochemical properties.
