Effect of andrographolide on phosphatases activity and cytotoxicity against Spodoptera litura

E Edwin; P Vasantha-Srinivasan; A Thanigaivel; A Ponsankar; S Selin-Rani; K Kalaivani; WB Hunter; V Duraipandiyan; NA AlDhabi

Invertebrate Survival Journal (May 2016)

Effect of andrographolide on phosphatases activity and cytotoxicity against Spodoptera litura

E Edwin,
P Vasantha-Srinivasan,
A Thanigaivel,
A Ponsankar,
S Selin-Rani,
K Kalaivani,
WB Hunter,
V Duraipandiyan,
NA AlDhabi

Affiliations

E Edwin: Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
P Vasantha-Srinivasan: Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
A Thanigaivel: Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
A Ponsankar: Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
S Selin-Rani: Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
K Kalaivani: United States Department of Agriculture, ARS, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA
WB Hunter: Post Graduate and Research Department of Zoology, Sri Parasakthi College for Women,Courtrallam-627 802, Tirunelveli, Tamil Nadu, India.
V Duraipandiyan: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O.Box.2455, Riyadh 11451, Kingdom of Saudi Arabia
NA AlDhabi: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O.Box.2455, Riyadh 11451, Kingdom of Saudi Arabia

Journal volume & issue: Vol. 13
pp. 153 – 163

Abstract

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Andrographolide was isolated from ethanol extraction of Andrographis paniculata by column chromatography. Evaluation of larvicidal efficacy, enzymatic changes and cytotoxic activities against Spodoptera litura were conducted across a range of concentrations. The compound showed significant larvicidal activity between 5 - 25 ppm, post ingestion. Morphological deformities observed in larval-pupal stages. Enzymatic profiles were altered by reduction in acid phosphatase, ACP activity by 69.18 %, alkaline phosphatase, ALP activity 75.3 % and 74.9 % reduction in ATPase. Binding affinity to midgut epithelium cells suggests disintegration of cellular organelles observed was directly associated with ingestion of the compound. The results suggest that andrographolide has potential for development as a significant inhibitor of development against the pest Spodoptera litura.

biorational insecticides; HPLC; GC-Mass; toxicity; Noctuidae; histology

Published in Invertebrate Survival Journal

ISSN: 1824-307X (Print)
Publisher: ISJ
Country of publisher: Italy
LCC subjects: Science: Biology (General)
Website: http://www.isj.unimo.it/

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