Nipah Virus Infection Generates Ordered Structures in Cellulo
Cecilia Alejandra Vázquez,
Lina Widerspick,
Roland Thuenauer,
Carola Schneider,
Rudolph Reimer,
Pedro Neira,
Catherine Olal,
Michelle Heung,
Linda Niemetz,
Philip Lawrence,
Indre Kucinskaite-Kodze,
Lars Redecke,
Beatriz Escudero-Pérez
Affiliations
Cecilia Alejandra Vázquez
Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Lina Widerspick
WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
Roland Thuenauer
Technology Platform Microscopy and Image Analysis, Leibniz Institute of Virology (LIV), 20251 Hamburg, Germany
Carola Schneider
Technology Platform Microscopy and Image Analysis, Leibniz Institute of Virology (LIV), 20251 Hamburg, Germany
Rudolph Reimer
Technology Platform Microscopy and Image Analysis, Leibniz Institute of Virology (LIV), 20251 Hamburg, Germany
Pedro Neira
WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
Catherine Olal
WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
Michelle Heung
WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
Linda Niemetz
WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
Philip Lawrence
Science and Humanities Confluence Research Center (EA 1598), Catholic University of Lyon (UCLy), 69002 Lyon, France
Indre Kucinskaite-Kodze
Life Science Center, Vilnius University, Sauletekio 7, LT-10257 Vilnius, Lithuania
Lars Redecke
Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany
Beatriz Escudero-Pérez
WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
Nipah virus (NiV) is a zoonotic paramyxovirus with a fatality rate of up to 92% in humans. While several pathogenic mechanisms used by NiV to counteract host immune defense responses have been described, all of the processes that take place in cells during infection are not fully characterized. Here, we describe the formation of ordered intracellular structures during NiV infection. We observed that these structures are formed specifically during NiV infection, but not with other viruses from the same Mononegavirales order (namely Ebola virus) or from other orders such as Bunyavirales (Junín virus). We also determined the kinetics of the appearance of these structures and their cellular localization at the cellular periphery. Finally, we confirmed the presence of these NiV-specific ordered structures using structured illumination microscopy (SIM), as well as their localization by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and correlative light and electron microscopy (CLEM). Herein, we describe a cytopathogenic mechanism that provides a new insight into NiV biology. These newly described ordered structures could provide a target for novel antiviral approaches.
