Nature Communications (Jun 2020)
Durable and controlled depletion of neutrophils in mice
- Gael Boivin,
- Julien Faget,
- Pierre-Benoit Ancey,
- Aspasia Gkasti,
- Julie Mussard,
- Camilla Engblom,
- Christina Pfirschke,
- Caroline Contat,
- Justine Pascual,
- Jessica Vazquez,
- Nathalie Bendriss-Vermare,
- Christophe Caux,
- Marie-Catherine Vozenin,
- Mikael J. Pittet,
- Matthias Gunzer,
- Etienne Meylan
Affiliations
- Gael Boivin
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- Julien Faget
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- Pierre-Benoit Ancey
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- Aspasia Gkasti
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- Julie Mussard
- University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon
- Camilla Engblom
- Center for Systems Biology, Massachusetts General Hospital Research Institute and Harvard Medical School
- Christina Pfirschke
- Center for Systems Biology, Massachusetts General Hospital Research Institute and Harvard Medical School
- Caroline Contat
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- Justine Pascual
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- Jessica Vazquez
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- Nathalie Bendriss-Vermare
- University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon
- Christophe Caux
- University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon
- Marie-Catherine Vozenin
- Laboratory of Radiation Oncology, Department of Radiation Oncology, Department of Oncology, CHUV, Lausanne University Hospital and University of Lausanne
- Mikael J. Pittet
- Center for Systems Biology, Massachusetts General Hospital Research Institute and Harvard Medical School
- Matthias Gunzer
- Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg–Essen
- Etienne Meylan
- Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne
- DOI
- https://doi.org/10.1038/s41467-020-16596-9
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 9
Abstract
Anti-Ly6G or ant-Gr1 antibodies are commonly used to deplete neutrophils in vivo. Here the authors provide mechanistic insight into why these approaches may not specifically or durably reduce the number of neutrophils in mice, and also present a new method that overcomes these limitations to have potentially wide applicability in experimental studies.
