Overexpression of TTRAP inhibits cell growth and induces apoptosis in osteosarcoma cells

Abstract

TTRAP is a multi-functional protein that is involved in multipleaspects of cellular functions including cell proliferation,apoptosis and the repair of DNA damage. Here, we demonstratedthat the lentivirus-mediated overexpression of TTRAPsignificantly inhibited cell growth and induced apoptosis inosteosarcoma cells. The ectopic TTRAP suppressed the growthand colony formation capacity of two osteosarcoma cell lines,U2OS and Saos-2. Cell apoptosis was induced in U2OS cellsand the cell cycle was arrested at G2/M phase in Saos-2 cells.Exogenous expression of TTRAP in serum-starved U2OS andSaos-2 cells induced an increase in caspase-3/-7 activity and adecrease in cyclin B1 expression. In comparison with wild-typeTTRAP, mutations in the 5'-tyrosyl-DNA phosphodiesteraseactivity of TTRAP, in particular TTRAPE152A, showed decreasedinhibitory activity on cell growth. These results may aid inclarifying the physiological functions of TTRAP, especially itsroles in the regulation of cell growth and tumorigenesis. [BMBReports 2013; 46(2): 113-118]

Keywords

• Apoptosis
• Growth inhibition
• Osteosarcoma
• TTRAP
• Tyrosyl DNA phosphodiesterase