A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity

Chun Liu; Pedro Medina; Dilip Thomas; Ian Y. Chen; Karim Sallam; Danish Sayed; Nazish Sayed

STAR Protocols (Jun 2021)

A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity

Chun Liu,
Pedro Medina,
Dilip Thomas,
Ian Y. Chen,
Karim Sallam,
Danish Sayed,
Nazish Sayed

Affiliations

Chun Liu: Stanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Medicine, Stanford University, Stanford, CA 94305, USA; Corresponding author
Pedro Medina: Stanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA
Dilip Thomas: Stanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Medicine, Stanford University, Stanford, CA 94305, USA
Ian Y. Chen: Stanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Medicine, Stanford University, Stanford, CA 94305, USA
Karim Sallam: Stanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Medicine, Stanford University, Stanford, CA 94305, USA
Danish Sayed: Department of Cell Biology and Molecular Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103, USA
Nazish Sayed: Stanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Medicine, Stanford University, Stanford, CA 94305, USA; Department of Surgery, Division of Vascular Surgery, Stanford University, Stanford, CA 94305, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 2
p. 100556

Abstract

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Summary: Endothelial cells (ECs) have emerged as key pathogenic players in cardiac disease due to their proximity with cardiomyocytes. Induced pluripotent stem cells (iPSCs) have been employed to generate ECs. However, it may be more clinically relevant to transdifferentiate fibroblasts into ECs directly without introducing pluripotent or virally driven transcription factors. Here, we present a protocol that describes the direct conversion of human cardiac fibroblasts into ECs by leveraging the innate immune system. Our protocol produces bona fide human ECs with 95%–98% purity by first passage.For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Sayed et al. (2015).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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