Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice

Abstract

BRV and GVBaV are the most important viruses affecting currant production worldwide. The subject of this work was to study genetic variability of Blackcurrant reversion virus (BRV) and Gooseberry vein banding associated virus (GVBaV) in the Czech Republic using 3’UTR and ORF 3 regions, respectively. In total, 155 sequences were obtained (74 for BRV; 81 for GVBaV) from a set of 103 BRV and 82 GVBaV RT-PCR-positive samples. Based on phylogenetic trees created in a MEGA X program, 38 different BRV genotypes and 30 GVBaV genotypes were defined. In both viruses, genotypes could be divided into two main clusters (A and B), and further subdivided into A1 and A2 subgroups, and B1 and B2 subgroups for BRV only. The most common BRV genotype was B2.16 (33.8 % of BRV-infected plants), and the GVBaV genotype B.4 (26.3 % of GVBaV-infected plants). Overall, an intraspecies variability in BRV was higher than in GVBaV that was generally low. BRV was found most frequently in production fields (84.2 % positive plants), while GVBaV was identified predominantly in non-production plantings. Black currant was the most frequent host of BRV, while GVBaV was mostly identified in red and white currants.

Keywords

• brv
• gvbav
• currant
• rt-pcr