Aqueous Humor Levels of Cytokines and Growth Factors in Patients with Congenital Aniridia

Abstract

Purpose. To study the concentration of cytokines and growth factors in the aqueous humor of patients with congenital aniridia.Patients and methods. The study comprised 10 patients (10 eyes) with congenital aniridia and 10 patients (10 eyes) with agerelated cataract. Aqueous humor was collected at the beginning of routine cataract surgery before the primary incision was created. A concentration of 27 cytokines and the transforming growth factor-β2 (TGF- β2) was determined using a Bio-Plex Pro™ Human Cytokine 27-plex Assay and Bio-Plex Pro™ and TGFβ 3-plex Assay kit by flow-through fluorometry on a Bio-Rad MAGPIX (Luminex Corp. Austin). Intergroup comparisons were conducted using the Mann-Whitney U test. Results. A comparative analysis showed a statistically significant increase in the concentration of IL-7 (p = 0.004), IL-15 (p = 0.008) and a decrease in the content of IL-9 (p = 0.008), IL-10 (p = 0.034) and GM-CSF (p = 0.045) in patients with congenital aniridia compared with the age-related cataract group. Inflammatory mediators (IL-1β, IL-6, IL-8, IL-12, IL-17A, MCP-1) and vascular endothelial growth factor (VEGF) in the aniridia group did not statistically differ from the comparison group.Conclusion. Statistically significant difference was detected in IL-7, IL-9, IL-10, IL-15, GM-CSF concentrations in the aqueous humor in patients with congenital aniridia when comparing to non aniridic patients with age-related cataract. The other cytokines with proinflammatory, angiogenic and profibrotic properties did not significantly differ from the comparison group.

Keywords

• congenital aniridia
• aniridic fibrosis syndrome
• cytokine
• aqueous humor