A quaternary tetramer assembly inhibits the deubiquitinating activity of USP25

Bing Liu; Marta Sureda-Gómez; Yang Zhen; Virginia Amador; David Reverter

doi:10.1038/s41467-018-07510-5

Nature Communications (Nov 2018)

A quaternary tetramer assembly inhibits the deubiquitinating activity of USP25

Bing Liu,
Marta Sureda-Gómez,
Yang Zhen,
Virginia Amador,
David Reverter

Affiliations

Bing Liu: Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona
Marta Sureda-Gómez: Institut de Investigacions Biomèdiques Agustí Pi i Sunyer (IDIBABS)
Yang Zhen: Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona
Virginia Amador: Institut de Investigacions Biomèdiques Agustí Pi i Sunyer (IDIBABS)
David Reverter: Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona

DOI: https://doi.org/10.1038/s41467-018-07510-5
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

Read online

USP25 is a deubiquitinating enzyme and a positive regulator of Wnt/β-catenin signaling. Here the authors present the crystal structure of USP25 in a tetrameric inactive state and their biochemical and kinetic assays support an USP25 autoinhibitory mechanism that is mediated through a dimer to tetramerization transition.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal