Multi-Omics Analyses Reveal Systemic Insights into Maize Vivipary
Yiru Wang,
Junli Zhang,
Minghao Sun,
Cheng He,
Ke Yu,
Bing Zhao,
Rui Li,
Jian Li,
Zongying Yang,
Xiao Wang,
Haiyang Duan,
Junjie Fu,
Sanzhen Liu,
Xuebin Zhang,
Jun Zheng
Affiliations
Yiru Wang
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Junli Zhang
State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475000, China
Minghao Sun
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Cheng He
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
Ke Yu
State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475000, China
Bing Zhao
State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475000, China
Rui Li
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jian Li
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zongying Yang
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xiao Wang
State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475000, China
Haiyang Duan
State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475000, China
Junjie Fu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Sanzhen Liu
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
Xuebin Zhang
State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475000, China
Jun Zheng
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Maize vivipary, precocious seed germination on the ear, affects yield and seed quality. The application of multi-omics approaches, such as transcriptomics or metabolomics, to classic vivipary mutants can potentially reveal the underlying mechanism. Seven maize vivipary mutants were selected for transcriptomic and metabolomic analyses. A suite of transporters and transcription factors were found to be upregulated in all mutants, indicating that their functions are required during seed germination. Moreover, vivipary mutants exhibited a uniform expression pattern of genes related to abscisic acid (ABA) biosynthesis, gibberellin (GA) biosynthesis, and ABA core signaling. NCED4 (Zm00001d007876), which is involved in ABA biosynthesis, was markedly downregulated and GA3ox (Zm00001d039634) was upregulated in all vivipary mutants, indicating antagonism between these two phytohormones. The ABA core signaling components (PYL-ABI1-SnRK2-ABI3) were affected in most of the mutants, but the expression of these genes was not significantly different between the vp8 mutant and wild-type seeds. Metabolomics analysis integrated with co-expression network analysis identified unique metabolites, their corresponding pathways, and the gene networks affected by each individual mutation. Collectively, our multi-omics analyses characterized the transcriptional and metabolic landscape during vivipary, providing a valuable resource for improving seed quality.
