Antimicrobial and Anti-Proliferative Effects of Skin Mucus Derived from Dasyatis pastinaca (Linnaeus, 1758)

Virginia Fuochi; Giovanni Li Volti; Giuseppina Camiolo; Francesco Tiralongo; Cesarina Giallongo; Alfio Distefano; Giulio Petronio Petronio; Ignazio Barbagallo; Maria Viola; Pio Maria Furneri; Michelino Di Rosa; Roberto Avola; Daniele Tibullo

doi:10.3390/md15110342

Marine Drugs (Nov 2017)

Antimicrobial and Anti-Proliferative Effects of Skin Mucus Derived from Dasyatis pastinaca (Linnaeus, 1758)

Virginia Fuochi,
Giovanni Li Volti,
Giuseppina Camiolo,
Francesco Tiralongo,
Cesarina Giallongo,
Alfio Distefano,
Giulio Petronio Petronio,
Ignazio Barbagallo,
Maria Viola,
Pio Maria Furneri,
Michelino Di Rosa,
Roberto Avola,
Daniele Tibullo

Affiliations

Virginia Fuochi: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Giovanni Li Volti: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Giuseppina Camiolo: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Francesco Tiralongo: Ente Fauna Marina Mediterranea, Avola, Siracusa 96012, Italy
Cesarina Giallongo: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Alfio Distefano: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Giulio Petronio Petronio: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Ignazio Barbagallo: Department of Drug Sciences, University of Catania, Catania 95125, Italy
Maria Viola: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Pio Maria Furneri: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Michelino Di Rosa: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Roberto Avola: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy
Daniele Tibullo: Department of Biomedical and Biotechnological Sciences, University of Catania, Catania 95124, Italy

DOI: https://doi.org/10.3390/md15110342
Journal volume & issue: Vol. 15, no. 11
p. 342

Abstract

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Resistance to chemotherapy occurs in various diseases (i.e., cancer and infection), and for this reason, both are very difficult to treat. Therefore, novel antimicrobial and chemotherapic drugs are needed for effective antibiotic therapy. The aim of the present study was to assess the antimicrobial and anti-proliferative effects of skin mucus derived from Dasyatis pastinaca (Linnaeus, 1758). Our results showed that skin mucus exhibited a significant and specific antibacterial activity against Gram-negative bacteria but not against Gram-positive bacteria. Furthermore, we also observed a significant antifungal activity against some strains of Candida spp. Concerning anti-proliferative activity, we showed that fish mucus was specifically toxic for acute leukemia cells (HL60) with an inhibition of proliferation in a dose dependent manner (about 52% at 1000 μg/mL of fish skin mucous, FSM). Moreover, we did not observe effects in healthy cells, in neuroblastoma cells (SH-SY5Y), and multiple myeloma cell lines (MM1, U266). Finally, it exhibited strong expression and activity of chitinase which may be responsible, at least in part, for the aforementioned results.

Published in Marine Drugs

ISSN: 1660-3397 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/marinedrugs

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