RADIOSENSITIVITY OF HELA CELLS INFECTED WITH POLIO VIRUS IRRADIATED BY CO 60

Abstract

BACKGROUND AND OBJECTIVE: The main purpose of radiotherapy is exposing enough dose to tumoral volume and protecting the normal tissues around it. Tumor dose for each fraction in radiotherapy prescribes with tumoral tissue consideration. Since viral diseases can affect cell radiosensitivity, this study was done to evaluate the radiosensitivity of tumoral cell that infected with virus. METHODS: In this study the radiosensitivity of HeLa cell with and without the viral agent of polio disease was obtained and compared after gamma radiation of cobalt 60. Different polio vaccine was used to infect cell with polio virus. Finally calculated the percentage of plating efficiency and relative survival at radiated and control cells with colonies countering. FINDINGS: Results implicate that cell death increases up to 20-30% for contaminating with low concentrations of polio virus, 30-40% for moderate concentrations and 70-90% for high concentrations after 2Gy irradiation by cobalt 60. CONCLUSION: Results of this study showed that existing of viral disease in tumoral region can increase radiosensitivity of tumoral cells. Therefore non cancer disease in region under irradiation should be noticed to prescribing dose fraction in radiotherapy of cancers.

Keywords

• radiosensitivity
• hela cells
• polio virus