Nature Communications (Oct 2019)
Genetic modification of primary human B cells to model high-grade lymphoma
- Rebecca Caeser,
- Miriam Di Re,
- Joanna A. Krupka,
- Jie Gao,
- Maribel Lara-Chica,
- João M. L. Dias,
- Susanna L. Cooke,
- Rachel Fenner,
- Zelvera Usheva,
- Hendrik F. P. Runge,
- Philip A. Beer,
- Hesham Eldaly,
- Hyo-Kyung Pak,
- Chan-Sik Park,
- George S. Vassiliou,
- Brian J. P. Huntly,
- Annalisa Mupo,
- Rachael J. M. Bashford-Rogers,
- Daniel J. Hodson
Affiliations
- Rebecca Caeser
- Wellcome MRC Cambridge Stem Cell Institute
- Miriam Di Re
- Wellcome MRC Cambridge Stem Cell Institute
- Joanna A. Krupka
- Wellcome MRC Cambridge Stem Cell Institute
- Jie Gao
- Wellcome MRC Cambridge Stem Cell Institute
- Maribel Lara-Chica
- Cancer Molecular Diagnostics Laboratory (CMDL), Department of Haematology, University of Cambridge
- João M. L. Dias
- Cancer Molecular Diagnostics Laboratory (CMDL), Department of Haematology, University of Cambridge
- Susanna L. Cooke
- Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow
- Rachel Fenner
- Wellcome MRC Cambridge Stem Cell Institute
- Zelvera Usheva
- Wellcome MRC Cambridge Stem Cell Institute
- Hendrik F. P. Runge
- Wellcome MRC Cambridge Stem Cell Institute
- Philip A. Beer
- Wellcome Sanger Institute, Wellcome Genome Campus
- Hesham Eldaly
- Department of Pathology, Cambridge University Hospitals
- Hyo-Kyung Pak
- Department of Pathology, University of Ulsan College of Medicine, Asan Medical Centre
- Chan-Sik Park
- Department of Pathology, University of Ulsan College of Medicine, Asan Medical Centre
- George S. Vassiliou
- Wellcome MRC Cambridge Stem Cell Institute
- Brian J. P. Huntly
- Wellcome MRC Cambridge Stem Cell Institute
- Annalisa Mupo
- Cancer Molecular Diagnostics Laboratory (CMDL), Department of Haematology, University of Cambridge
- Rachael J. M. Bashford-Rogers
- Wellcome Centre for Human Genetics
- Daniel J. Hodson
- Wellcome MRC Cambridge Stem Cell Institute
- DOI
- https://doi.org/10.1038/s41467-019-12494-x
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 16
Abstract
A dearth of adequate preclinical models to faithfully mimic diffuse large B-cell lymphoma has hampered the identification of driver genes. Here, the authors present a co-culture system that enables ex vivo expansion, viral transduction and transformation of primary human germinal center B cells.
