Breast Cancer Cell Destruction Using Individually Encapsulated Cytotoxic T Lymphocytes in Polyelectrolyte Layer Coated with Dual Nanoparticles in Combination with Magnetic Exposure and Laser Irradiation

Abstract

ABSTRACT Cell encapsulation can protect cells from the host immune system. It is well‐known that cytotoxic T lymphocytes (CTLs) can destroy cancer cells. In this study, we individually encapsulated CTLs in a cationic polyelectrolyte in combination with two types of nanomaterials: magnetic nanoparticles (MNPs) and gold nanorods (GNRs) conjugated to anti‐human epidermal growth factor receptor‐2 (HER2) antibodies referred to as CTLs/mMNPs/PAH/GNRs@HER2. When MCF‐7 breast cancer cells were co‐cultured with CTLs/mMNPs/PAH/GNRs@HER2 and subjected to a magnetic field and laser irradiation, the viability of MCF‐7 cells was significantly decreased to 42.98 ± 0.57% in comparison to MCF‐7 cells without any treatment or MCF‐7 cells co‐cultured with CTLs (51.52 ± 0.57%). This can be a novel strategy for cancer treatment using encapsulated CTLs in polyelectrolytes and dual nanomaterials in combination with magnetic exposure and laser irradiation.

Keywords

• breast cancer destruction
• cell encapsulation
• gold nanorod
• granzyme B and perforin
• magnetic nanoparticle