Marine Bacterium <i>Vibrio</i> sp. CB1-14 Produces Guanidine Alkaloid 6-<i>epi</i>-Monanchorin, Previously Isolated from Marine Polychaete and Sponges
Tatyana Makarieva,
Larisa Shubina,
Valeria Kurilenko,
Marina Isaeva,
Nadezhda Chernysheva,
Roman Popov,
Evgeniya Bystritskaya,
Pavel Dmitrenok,
Valentin Stonik
Affiliations
Tatyana Makarieva
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Larisa Shubina
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Valeria Kurilenko
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Marina Isaeva
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Nadezhda Chernysheva
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Roman Popov
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Evgeniya Bystritskaya
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Pavel Dmitrenok
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Valentin Stonik
G.B. Elyakov Pacific Institute of Bioorganic Chemistry (PIBOC), Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia
Twenty-three bacterial strains were isolated from the secreted mucus trapping net of the marine polychaete Chaetopterus variopedatus (phylum Annelida) and twenty strains were identified using 16S rRNA gene analysis. Strain CB1-14 was recognized as a new species of the genus Vibrio using the eight-gene multilocus sequence analysis (MLSA) and genome sequences of nineteen type Vibrio strains. This Vibrio sp. was cultured, and 6-epi-monanchorin (2), previously isolated from the polychaete and two sponge species, was found in the cells and culture broth. The presence of the 6-epi-monanchorin was confirmed by its isolation followed by 1H NMR and HRESIMS analysis. These results showed the microbial origin of the bicyclic guanidine alkaloid 2 in C. variopedatus.
