Molecular cytogenetic identification and nutritional composition evaluation of newly synthesized Triticum turgidum-Triticum boeoticum amphiploids (AABBAbAb)

Xin Liu; Xin Liu; Xiaomei Jiang; Xiaomei Jiang; Junqing Zhang; Junqing Zhang; Hong Ye; Hong Ye; Mang Shen; Mang Shen; Lei Wu; Lei Wu; Yongping Miao; Yongping Miao; Longyu Chen; Longyu Chen; Ke Zhou; Ke Zhou; Ming Hao; Bo Jiang; Lin Huang; Lin Huang; Shunzong Ning; Xuejiao Chen; Xue Chen; Dengcai Liu; Dengcai Liu; Lianquan Zhang; Lianquan Zhang

doi:10.3389/fpls.2023.1285847

Frontiers in Plant Science (Dec 2023)

Molecular cytogenetic identification and nutritional composition evaluation of newly synthesized Triticum turgidum-Triticum boeoticum amphiploids (AABBAbAb)

Xin Liu,
Xin Liu,
Xiaomei Jiang,
Xiaomei Jiang,
Junqing Zhang,
Junqing Zhang,
Hong Ye,
Hong Ye,
Mang Shen,
Mang Shen,
Lei Wu,
Lei Wu,
Yongping Miao,
Yongping Miao,
Longyu Chen,
Longyu Chen,
Ke Zhou,
Ke Zhou,
Ming Hao,
Bo Jiang,
Lin Huang,
Lin Huang,
Shunzong Ning,
Xuejiao Chen,
Xue Chen,
Dengcai Liu,
Dengcai Liu,
Lianquan Zhang,
Lianquan Zhang

Affiliations

Xin Liu: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Xin Liu: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Xiaomei Jiang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Xiaomei Jiang: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Junqing Zhang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Junqing Zhang: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Hong Ye: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Hong Ye: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Mang Shen: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Mang Shen: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Lei Wu: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Lei Wu: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Yongping Miao: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Yongping Miao: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Longyu Chen: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Longyu Chen: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Ke Zhou: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Ke Zhou: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Ming Hao: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Bo Jiang: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Lin Huang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Lin Huang: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Shunzong Ning: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Xuejiao Chen: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Xue Chen: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Dengcai Liu: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Dengcai Liu: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
Lianquan Zhang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
Lianquan Zhang: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China

DOI: https://doi.org/10.3389/fpls.2023.1285847
Journal volume & issue: Vol. 14

Abstract

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Triticum boeoticum Boiss. (AbAb, 2n = 2x = 14) is a wheat-related species with the blue aleurone trait. In this study, 18 synthetic Triticum turgidum-Triticum boeoticum amphiploids were identified, which were derived from crosses between T. boeoticum and T. turgidum. Three probes (Oligo-pTa535, Oligo-pSc119.2, and Oligo-pTa713) for multicolor fluorescence in situ hybridization (mc-FISH) were combined with genomic in situ hybridization (GISH) to identify chromosomal composition. Seven nutritional indices (anthocyanins, protein, total essential amino acids TEAA, Fe, Zn, Mn and Cu) were measured, and the nutritional components of 18 synthetic amphiploids were comprehensively ranked by principal component analysis (PCA). The results showed that all three synthetic amphiploids used for cytological identification contained 42 chromosomes, including 14 A, 14 B, and 14 Ab chromosomes. The average anthocyanin content was 82.830 μg/g to 207.606 μg/g in the whole meal of the 17 blue-grained lines (Syn-ABAb-1 to Syn-ABAb-17), which was obviously higher than that in the yellow-grained line Syn-ABAb-18 (6.346 μg/g). The crude protein content was between 154.406 and 180.517 g/kg, and the EAA content was 40.193-63.558 mg/g. The Fe, Zn, Mn and Cu levels in the 17 blue-grained lines were 60.55 to 97.41 mg/kg, 60.55-97.41 mg/kg, 35.11 to 65.20 mg/kg and 5.74 to 7.22 mg/kg, respectively, which were higher than those in the yellow-grained line. The contribution of the first three principal components reached 84%. The first principal component was mainly anthocyanins, Fe, Zn and Mn. The second principal component contained protein and amino acids, and the third component contained only Cu. The top 5 Triticum turgidum-Triticum boeoticum amphiploids were Syn-ABAb-11, Syn-ABAb-17, Syn-ABAb-5, Syn-ABAb-8 and Syn-ABAb-4. These amphidiploids exhibited the potential to serve as candidates for hybridization with common wheat, as indicated by comprehensive score rankings, toward enhancing the nutritional quality of wheat.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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