QUALITATIVE CHEMICAL PROFILE AND BIOLOGICAL ACTIVITY OF BROWNEA GRANDICEPS JACQ. (FABACEAE) LEAVES AND STEMS EXTRACTS

Abstract

The hexane, dichloromethane, and methanol extracts obtained from aerial parts of Brownea grandiceps Jacq (Fabaceae) were studied to determine the antioxidant and tick control activity. The qualitative chemical composition showed the presence of sterols in hexane and dichloromethane extracts of leaves while in methanol extract were observed alkaloids, sterols, polyphenols, tannins, and flavonoids. Furthermore, hexane extract of stems revealed the presence of terpenes; dichloromethane extract showed sterols whereas methanol extract exhibited the presence of alkaloids, polyphenols, tannins, and flavonoids. On the other hand, phenolic compounds were observed in methanol extract of leaves and stems of this species with values of 0.888 and 0.886 μg GAE/mg of extract, respectively. Regarding the scavenging capacity measured the by DPPH method, methanol extracts of leaves and stems showed the highest value at a concentration of 0.5 mg/mL with percentages of inhibition (% I) of 89.2 and 88.9 respectively. However, a much lower activity was observed with hexane extract of the leaves with a % I of 20.1 at the same concentration. Regarding inverse of effective concentration assay, methanol extract of leaves and stems showed values of 10.0 and 7.14 mL/mg. In addition, methanol extracts of leaves and stems of B. grandiceps did not show activity on the larvae of Riphicephalus microplus.

Keywords

• brownea grandiceps jacq
• fabaceae
• dpph
• phenols content
• riphicephalus microplus