Antimicrobial Nanostructured Bioactive Coating Based on Fe3O4 and Patchouli Oil for Wound Dressing
Marius Rădulescu,
Ecaterina Andronescu,
Alina Maria Holban,
Bogdan Stefan Vasile,
Florin Iordache,
Laurențiu Mogoantă,
George Dan Mogoșanu,
Alexandru Mihai Grumezescu,
Mihaela Georgescu,
Mariana Carmen Chifiriuc
Affiliations
Marius Rădulescu
Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, Bucharest 011061, Romania
Ecaterina Andronescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, Bucharest 011061, Romania
Alina Maria Holban
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, Bucharest 011061, Romania
Bogdan Stefan Vasile
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, Bucharest 011061, Romania
Florin Iordache
Department of Fetal and Adult Stem Cell Therapy, Institute of Cellular Biology and Pathology of Romanian Academy, 8, B.P. Hasdeu, Bucharest 050568, Romania
Laurențiu Mogoantă
Research Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, Petru Rares Street, No. 2, Craiova 200349, Romania
George Dan Mogoșanu
Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Petru Rares Street, No. 2, Craiova 200349, Romania
Alexandru Mihai Grumezescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, Bucharest 011061, Romania
Mihaela Georgescu
Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, Bucharest 77206, Romania
Mariana Carmen Chifiriuc
Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, Bucharest 77206, Romania
The aim of this study was to develop a biocompatible coating for wound dressings, containing iron oxide nanoparticles functionalized with patchouli essential oil in order to obtain improved antimicrobial properties able to prevent biofilm development and consecutive associated infections. The bioactive coating was prepared by the co-precipitation of a precursor in an alkaline solution of patchouli oil. The prepared surface was characterized by XRD (X ray diffraction), TEM (transmission electron microscopy), SAED (selected area diffraction), SEM (scanning electron microscopy) and FT-IR (Fourier transform infrared spectroscopy). The bioevaluation of the obtained coating consisted in antimicrobial, as well as in vitro and in vivo biocompatibility and biodistribution assays. The obtained coating revealed a strong anti-biofilm activity maintained up to 72 h, as well as a low cytotoxicity on mammalian cells and a good biodistribution after intraperitoneal injection in mice. These results demonstrate the promising potential of the respective coatings for the management of wound infections and for the development of soft materials with improved resistance to microbial colonization.
