Effect of Arabinoxylan from Wastewater Generated during Vital Wheat Gluten Production on Liver Metabolism in Type 2 Diabetic Mice
Denglin Luo,
Xingguo Li,
Mengyuan Geng,
Yunhui Zhang,
Honglin Lan,
Jiale Li,
Caili Qi,
Zhouya Bai,
Jihong Huang
Affiliations
Denglin Luo
Food and Pharmacy College, Xuchang University, Xuchang 461000, China
Xingguo Li
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Mengyuan Geng
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Yunhui Zhang
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Honglin Lan
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Jiale Li
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Caili Qi
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Zhouya Bai
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Jihong Huang
Food and Pharmacy College, Xuchang University, Xuchang 461000, China
Arabinoxylan (AX) is a dietary fiber that has been proven to have a significant antidiabetic effect. Liver metabolic disorders frequently coincide with the development of type 2 diabetes, but research on the hepatoprotective effects of AX in type 2 diabetic mice is lacking. As AX is abundant in the wastewater produced during vital wheat gluten protein production, this study used it as a raw material to evaluate its protective effect on liver function. The study employed an AX intervention in type 2 diabetic mice induced by a high-fat diet combined with streptozotocin and collected serum and liver tissue samples after 4 weeks. Serum and liver function indicators were measured using an automatic biochemistry analysis apparatus, and liver fat accumulation was observed using oil red O staining. Nontargeted metabolomics analysis of liver tissues was conducted using UHPLC-MS/MS. The results showed that AX significantly improved liver function indicators and histopathological damage, and regulated liver metabolic disorders by improving the differential metabolites of pantothenate and CoA biosynthesis, as well as purine metabolism. This study demonstrated that AX may exert a significant hepatoprotective effect by regulating metabolic disorders.
