Frontiers in Cell and Developmental Biology (Sep 2021)

The Dynamic Changes of Transcription Factors During the Development Processes of Human Biparental and Uniparental Embryos

  • Chenxi Zhang,
  • Chenxi Zhang,
  • Chenxi Zhang,
  • Conghui Li,
  • Conghui Li,
  • Conghui Li,
  • Conghui Li,
  • Conghui Li,
  • Ling Yang,
  • Ling Yang,
  • Ling Yang,
  • Ling Yang,
  • Lizhi Leng,
  • Lizhi Leng,
  • Lizhi Leng,
  • Dragomirka Jovic,
  • Dragomirka Jovic,
  • Jun Wang,
  • Jun Wang,
  • Jun Wang,
  • Fang Fang,
  • Fang Fang,
  • Guibo Li,
  • Guibo Li,
  • Depeng Zhao,
  • Xuemei Li,
  • Lin Lin,
  • Yonglun Luo,
  • Yonglun Luo,
  • Yonglun Luo,
  • Yonglun Luo,
  • Yonglun Luo,
  • Yonglun Luo,
  • Yonglun Luo,
  • Lars Bolund,
  • Lars Bolund,
  • Lars Bolund,
  • Lars Bolund,
  • Jinrong Huang,
  • Jinrong Huang,
  • Jinrong Huang,
  • Jinrong Huang,
  • Jinrong Huang,
  • Ge Lin,
  • Ge Lin,
  • Ge Lin,
  • Fengping Xu,
  • Fengping Xu,
  • Fengping Xu,
  • Fengping Xu,
  • Fengping Xu,
  • Fengping Xu

DOI
https://doi.org/10.3389/fcell.2021.709498
Journal volume & issue
Vol. 9

Abstract

Read online

Previous studies have revealed that transcription factors (TFs) play important roles in biparental (BI) early human embryogenesis. However, the contribution of TFs during early uniparental embryo development is still largely unknown. Here we systematically studied the expression profiles of transcription factors in early embryonic development and revealed the dynamic changes of TFs in human biparental and uniparental embryogenesis by single-cell RNA sequencing (scRNA-seq). In general, the TF expression model of uniparental embryos showed a high degree of conformity with biparental embryos. The detailed network analysis of three different types of embryos identified that 10 out of 17 hub TFs were shared or specifically owned, such as ZNF480, ZNF581, PHB, and POU5F1, were four shared TFs, ZFN534, GTF3A, ZNF771, TEAD4, and LIN28A, were androgenic (AG) specific TFs, and ZFP42 was the only one parthenogenetic (PG) specific TF. All the four shared TFs were validated using human embryonic stem cell (hESC) differentiation experiments; most of their target genes are responsible for stem cell maintenance and differentiation. We also found that Zf-C2H2, HMG, and MYB were three dominant transcription factor families that appeared in early embryogenesis. Altogether, our work provides a comprehensive regulatory framework and better understanding of TF function in human biparental and uniparental embryogenesis.

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