Nitrogen Fertilizer Amendment Alter the Bacterial Community Structure in the Rhizosphere of Rice (Oryza sativa L.) and Improve Crop Yield

Jun Chen; Jun Chen; Jun Chen; Yasir Arafat; Yasir Arafat; Yasir Arafat; Israr Ud Din; Bo Yang; Bo Yang; Bo Yang; Liuting Zhou; Liuting Zhou; Juanying Wang; Juanying Wang; Puleng Letuma; Puleng Letuma; Hongmiao Wu; Hongmiao Wu; Hongmiao Wu; Xianjin Qin; Xianjin Qin; Linkun Wu; Linkun Wu; Linkun Wu; Sheng Lin; Sheng Lin; Zhixing Zhang; Zhixing Zhang; Zhixing Zhang; Wenxiong Lin; Wenxiong Lin; Wenxiong Lin

doi:10.3389/fmicb.2019.02623

Frontiers in Microbiology (Nov 2019)

Nitrogen Fertilizer Amendment Alter the Bacterial Community Structure in the Rhizosphere of Rice (Oryza sativa L.) and Improve Crop Yield

Jun Chen,
Jun Chen,
Jun Chen,
Yasir Arafat,
Yasir Arafat,
Yasir Arafat,
Israr Ud Din,
Bo Yang,
Bo Yang,
Bo Yang,
Liuting Zhou,
Liuting Zhou,
Juanying Wang,
Juanying Wang,
Puleng Letuma,
Puleng Letuma,
Hongmiao Wu,
Hongmiao Wu,
Hongmiao Wu,
Xianjin Qin,
Xianjin Qin,
Linkun Wu,
Linkun Wu,
Linkun Wu,
Sheng Lin,
Sheng Lin,
Zhixing Zhang,
Zhixing Zhang,
Zhixing Zhang,
Wenxiong Lin,
Wenxiong Lin,
Wenxiong Lin

Affiliations

Jun Chen: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Jun Chen: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Jun Chen: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China
Yasir Arafat: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Yasir Arafat: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Yasir Arafat: Key Laboratory of Crop Genetic Breeding and Comprehensive Utilization of the Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China
Israr Ud Din: Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar, Pakistan
Bo Yang: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Bo Yang: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Bo Yang: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China
Liuting Zhou: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Liuting Zhou: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Juanying Wang: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Juanying Wang: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Puleng Letuma: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Puleng Letuma: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China
Hongmiao Wu: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Hongmiao Wu: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Hongmiao Wu: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China
Xianjin Qin: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Xianjin Qin: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China
Linkun Wu: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Linkun Wu: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Linkun Wu: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China
Sheng Lin: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Sheng Lin: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Zhixing Zhang: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Zhixing Zhang: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Zhixing Zhang: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China
Wenxiong Lin: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China
Wenxiong Lin: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China
Wenxiong Lin: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, China

DOI: https://doi.org/10.3389/fmicb.2019.02623
Journal volume & issue: Vol. 10

Abstract

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Availability of nitrogen (N) in soil changes the composition and activities of microbial community, which is critical for the processing of soil organic matter and health of crop plants. Inappropriate application of N fertilizer can alter the rhizosphere microbial community and disturb the soil N homeostasis. The goal of this study was to assess the effect of different ratio of N fertilizer at various early to late growth stages of rice, while keeping the total N supply constant on rice growth performance, microbial community structure, and soil protein expression in rice rhizosphere. Two different N regimes were applied, i.e., traditional N application (NT) consists of three sessions including 60, 30 and 10% at pre-transplanting, tillering and panicle initiation stages, respectively, while efficient N application (NF) comprises of four sessions, i.e., 30, 30, 30, and 10%), where the fourth session was extended to anthesis stage. Soil metaproteomics combined with Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to determine the rhizosphere biological process. Under NF application, soil enzymes, nitrogen utilization efficiency and rice yield were significantly higher compared to NT application. T-RFLP and qPCR analysis revealed differences in rice rhizosphere bacterial diversity and structure. NF significantly decreased the specific microbes related to denitrification, but opposite result was observed for bacteria associated with nitrification. Furthermore, soil metaproteomics analysis showed that 88.28% of the soil proteins were derived from microbes, 5.74% from plants, and 6.25% from fauna. Specifically, most of the identified microbial proteins were involved in carbohydrate, amino acid and protein metabolisms. Our experiments revealed that NF positively regulates the functioning of the rhizosphere ecosystem and further enabled us to put new insight into microbial communities and soil protein expression in rice rhizosphere.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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