Frontiers in Microbiology (2019-11-01)

Nitrogen Fertilizer Amendment Alter the Bacterial Community Structure in the Rhizosphere of Rice (Oryza sativa L.) and Improve Crop Yield

  • Jun Chen,
  • Jun Chen,
  • Jun Chen,
  • Yasir Arafat,
  • Yasir Arafat,
  • Yasir Arafat,
  • Israr Ud Din,
  • Bo Yang,
  • Bo Yang,
  • Bo Yang,
  • Liuting Zhou,
  • Liuting Zhou,
  • Juanying Wang,
  • Juanying Wang,
  • Puleng Letuma,
  • Puleng Letuma,
  • Hongmiao Wu,
  • Hongmiao Wu,
  • Hongmiao Wu,
  • Xianjin Qin,
  • Xianjin Qin,
  • Linkun Wu,
  • Linkun Wu,
  • Linkun Wu,
  • Sheng Lin,
  • Sheng Lin,
  • Zhixing Zhang,
  • Zhixing Zhang,
  • Zhixing Zhang,
  • Wenxiong Lin,
  • Wenxiong Lin,
  • Wenxiong Lin

DOI
https://doi.org/10.3389/fmicb.2019.02623
Journal volume & issue
Vol. 10

Abstract

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Availability of nitrogen (N) in soil changes the composition and activities of microbial community, which is critical for the processing of soil organic matter and health of crop plants. Inappropriate application of N fertilizer can alter the rhizosphere microbial community and disturb the soil N homeostasis. The goal of this study was to assess the effect of different ratio of N fertilizer at various early to late growth stages of rice, while keeping the total N supply constant on rice growth performance, microbial community structure, and soil protein expression in rice rhizosphere. Two different N regimes were applied, i.e., traditional N application (NT) consists of three sessions including 60, 30 and 10% at pre-transplanting, tillering and panicle initiation stages, respectively, while efficient N application (NF) comprises of four sessions, i.e., 30, 30, 30, and 10%), where the fourth session was extended to anthesis stage. Soil metaproteomics combined with Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to determine the rhizosphere biological process. Under NF application, soil enzymes, nitrogen utilization efficiency and rice yield were significantly higher compared to NT application. T-RFLP and qPCR analysis revealed differences in rice rhizosphere bacterial diversity and structure. NF significantly decreased the specific microbes related to denitrification, but opposite result was observed for bacteria associated with nitrification. Furthermore, soil metaproteomics analysis showed that 88.28% of the soil proteins were derived from microbes, 5.74% from plants, and 6.25% from fauna. Specifically, most of the identified microbial proteins were involved in carbohydrate, amino acid and protein metabolisms. Our experiments revealed that NF positively regulates the functioning of the rhizosphere ecosystem and further enabled us to put new insight into microbial communities and soil protein expression in rice rhizosphere.

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