Relationship of Metabolic Dysfunction-Associated Steatohepatitis-Related Hepatocellular Carcinoma with Oral and Intestinal Microbiota: A Cross-Sectional Pilot Study
Takaaki Matsui,
Toshiya Morozumi,
Yuko Yamamoto,
Takashi Kobayashi,
Ryo Takuma,
Masato Yoneda,
Asako Nogami,
Takaomi Kessoku,
Muneaki Tamura,
Yoshiaki Nomura,
Toru Takahashi,
Yohei Kamata,
Shuntaro Sugihara,
Kyoko Arai,
Masato Minabe,
Norio Aoyama,
Kenji Mitsudo,
Atsushi Nakajima,
Motohiro Komaki
Affiliations
Takaaki Matsui
Department of Periodontology, Faculty of Dentistry, Kanagawa Dental University, Yokosuka 238-8580, Japan
Toshiya Morozumi
Department of Periodontology, Faculty of Dentistry, Kanagawa Dental University, Yokosuka 238-8580, Japan
Yuko Yamamoto
Department of Dental Hygiene, Kanagawa Dental University, Junior College, Yokosuka 238-8580, Japan
Takashi Kobayashi
Department of Gastroenterology and Hepatology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Ryo Takuma
Department of Periodontology, Faculty of Dentistry, Kanagawa Dental University, Yokosuka 238-8580, Japan
Masato Yoneda
Department of Gastroenterology and Hepatology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Asako Nogami
Department of Gastroenterology and Hepatology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Takaomi Kessoku
Department of Gastroenterology and Hepatology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Muneaki Tamura
Department of Microbiology and Immunology, Nihon University School of Dentistry, Tokyo 101-8310, Japan
Yoshiaki Nomura
Institute of Photochemistry and Photofunctional Materials, University of Shanghai for Science and Technology, Shanghai 200093, China
Toru Takahashi
Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University, Saitama 362-0806, Japan
Yohei Kamata
Department of Advanced Periodontology, Faculty of Dentistry, Kanagawa Dental University, Yokohama 221-0835, Japan
Shuntaro Sugihara
Department of Periodontology, Faculty of Dentistry, Kanagawa Dental University, Yokosuka 238-8580, Japan
Kyoko Arai
Department of Endodontics, The Nippon Dental University School of Life Dentistry at Niigata, Niigata 951-8580, Japan
Masato Minabe
Bunkyo-Dori Dental Clinic, Chiba 263-0024, Japan
Norio Aoyama
Department of Education Planning, Kanagawa Dental University, Yokosuka 238-8580, Japan
Kenji Mitsudo
Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Atsushi Nakajima
Department of Gastroenterology and Hepatology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Motohiro Komaki
Department of Periodontology, Faculty of Dentistry, Kanagawa Dental University, Yokosuka 238-8580, Japan
Background and Objectives: The incidence of metabolic dysfunction-associated steatohepatitis (MASH)-related hepatocellular carcinoma (HCC) is increasing worldwide, alongside the epidemic of obesity and metabolic syndrome. Based on preliminary reports regarding the potential association of HCC and periodontitis, this study aimed to analyze the involvement of periodontal bacteria as well as the oral and intestinal bacterial flora in MASH-related HCC (MASH-HCC). Materials and Methods: Forty-one patients with MASH and nineteen with MASH-HCC participated in the study, completing survey questionnaires, undergoing periodontal examinations, and providing samples of saliva, mouth-rinsed water, feces, and peripheral blood. The oral and fecal microbiome profiles were analyzed by 16S ribosomal RNA sequencing. Bayesian network analysis was used to analyze the causation between various factors, including MASH-HCC, examinations, and bacteria. Results: The genus Fusobacterium had a significantly higher occupancy rate (p = 0.002) in the intestinal microflora of the MASH-HCC group compared to the MASH group. However, Butyricicoccus (p = 0.022) and Roseburia (p Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, and Prevotella intermedia in the saliva, as well as the genera Lactobacillus, Roseburia, Fusobacterium, Prevotella, Clostridium, Ruminococcus, Trabulsiella, and SMB53 in the intestine. Furthermore, P. gingivalis in the oral cavity directly affected the genera Lactobacillus and Streptococcus in the intestine. Conclusions: MASH-HCC directly affects periodontal pathogenic and intestinal bacteria, and P. gingivalis may affect the intestinal bacteria associated with gastrointestinal cancer.
