Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration

Selin Pars; Fernando Cristo; José M. Inácio; Graça Rosas; Isabel Marques Carreira; Joana Barbosa Melo; Patrícia Mendes; Duarte Saraiva Martins; Luís Pereira de Almeida; José Maio; Rui Anjos; José A. Belo

Stem Cell Research (May 2018)

Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration

Selin Pars,
Fernando Cristo,
José M. Inácio,
Graça Rosas,
Isabel Marques Carreira,
Joana Barbosa Melo,
Patrícia Mendes,
Duarte Saraiva Martins,
Luís Pereira de Almeida,
José Maio,
Rui Anjos,
José A. Belo

Affiliations

Selin Pars: Stem Cells and Development Laboratory, CEDOC, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal
Fernando Cristo: Stem Cells and Development Laboratory, CEDOC, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal
José M. Inácio: Stem Cells and Development Laboratory, CEDOC, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal
Graça Rosas: Stem Cells and Development Laboratory, CEDOC, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal
Isabel Marques Carreira: Cytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Coimbra, Portugal; CNC.IBILI Consortium, University of Coimbra, Coimbra, Portugal; CIMAGO - Center of Investigation on Environment Genetics and Oncobiology, Faculty of Medicine, University of Coimbra, Portugal
Joana Barbosa Melo: Cytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Coimbra, Portugal; CNC.IBILI Consortium, University of Coimbra, Coimbra, Portugal; CIMAGO - Center of Investigation on Environment Genetics and Oncobiology, Faculty of Medicine, University of Coimbra, Portugal
Patrícia Mendes: Departamento Materno-Infantil, Centro Hospital do Algarve, EPE, Faro, Portugal
Duarte Saraiva Martins: Hospital de Santa Cruz, Centro Hospitalar Lisboa Ocidental, Lisbon, Portugal
Luís Pereira de Almeida: CNC - Center for Neurosciences & Cell Biology, University of Coimbra, Coimbra, Portugal
José Maio: Departamento Materno-Infantil, Centro Hospital do Algarve, EPE, Faro, Portugal
Rui Anjos: Hospital de Santa Cruz, Centro Hospitalar Lisboa Ocidental, Lisbon, Portugal
José A. Belo: Stem Cells and Development Laboratory, CEDOC, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal; Corresponding author.

Journal volume & issue: Vol. 29
pp. 202 – 206

Abstract

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A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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