Chemical Components and Biological Activities of Essential Oils of <i>Mentha × piperita</i> L. from Field-Grown and Field-Acclimated after In Vitro Propagation Plants
Olga V. Shelepova,
Tatyana A. Dilovarova,
Alexander A. Gulevich,
Ludmila S. Olekhnovich,
Anna V. Shirokova,
Irina T. Ushakova,
Ekaterina V. Zhuravleva,
Ludmila N. Konovalova,
Ekaterina N. Baranova
Affiliations
Olga V. Shelepova
Plant Physiology and Immunity Laboratory, N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, Russia
Tatyana A. Dilovarova
Plant Cell Biology Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia
Alexander A. Gulevich
Plant Cell Engineering Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia
Ludmila S. Olekhnovich
Plant Physiology and Immunity Laboratory, N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, Russia
Anna V. Shirokova
Pungent and Aromatic Herbs Laboratory, Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSVC), 143072 Moscow, Russia
Irina T. Ushakova
Pungent and Aromatic Herbs Laboratory, Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSVC), 143072 Moscow, Russia
Ekaterina V. Zhuravleva
Federal State Budgetary Scientific Institution Belgorod Federal Agrarian Scientific Center of Russian Academy of Sciences, 308001 Belgorod, Russia
Ludmila N. Konovalova
Plant Recombinogenesis Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia
Ekaterina N. Baranova
Plant Cell Biology Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia
In this work, we studied in vitro propagation of three cultivars of Mentha × piperita L. Murashige and Skoog medium (MS) supplemented with 0.5 mg·L−1 BAP was the most optimal medium for micropropagation of the cultivars studied. The ability of peppermint plants field-acclimated after in vitro micropropagation to produce essential oils (EOs) was investigated. EO was obtained by hydrodistillation from dried leaves and flowering shoots from control (field grown) plants and plants acclimated in field conditions after in vitro propagation. The samples were collected at the first and second year of vegetation, and their chemical composition was investigated using gas chromatography-mass spectrometry (GC-MS). Differences were observed in the yield, as well as in the quantitative and qualitative composition of the EOs extracted from the control plants and field-acclimated plants after in vitro propagation. Menthol was the main component of the EO in control plants, while pulegone and menthone were dominant in the EO pattern in field-acclimated in vitro regenerants in the first year of the growing season. However, in the second year of vegetation, the content of the main EO components in field-acclimated peppermint plants was approximately the same as in control plants. The antioxidant activity of EOs extracted from field-acclimated after in vitro micropropagation plants was found to be the same as in control field-grown M. × piperita plants.
