Frontiers in Immunology (Jan 2022)

Multiplex Antibody Analysis of IgM, IgA and IgG to SARS-CoV-2 in Saliva and Serum From Infected Children and Their Close Contacts

  • Carlota Dobaño,
  • Carlota Dobaño,
  • Selena Alonso,
  • Marta Vidal,
  • Alfons Jiménez,
  • Alfons Jiménez,
  • Rocío Rubio,
  • Rebeca Santano,
  • Diana Barrios,
  • Gemma Pons Tomas,
  • María Melé Casas,
  • María Hernández García,
  • Mònica Girona-Alarcón,
  • Mònica Girona-Alarcón,
  • Laura Puyol,
  • Barbara Baro,
  • Pere Millat-Martínez,
  • Sara Ajanovic,
  • Núria Balanza,
  • Sara Arias,
  • Natalia Rodrigo Melero,
  • Carlo Carolis,
  • Aleix García-Miquel,
  • Elisenda Bonet-Carné,
  • Elisenda Bonet-Carné,
  • Elisenda Bonet-Carné,
  • Joana Claverol,
  • Joana Claverol,
  • Marta Cubells,
  • Marta Cubells,
  • Claudia Fortuny,
  • Claudia Fortuny,
  • Victoria Fumadó,
  • Victoria Fumadó,
  • Anna Codina,
  • Quique Bassat,
  • Quique Bassat,
  • Quique Bassat,
  • Quique Bassat,
  • Quique Bassat,
  • Carmen Muñoz-Almagro,
  • Carmen Muñoz-Almagro,
  • Carmen Muñoz-Almagro,
  • Carmen Muñoz-Almagro,
  • Mariona Fernández de Sevilla,
  • Mariona Fernández de Sevilla,
  • Mariona Fernández de Sevilla,
  • Eduard Gratacós,
  • Eduard Gratacós,
  • Eduard Gratacós,
  • Luis Izquierdo,
  • Luis Izquierdo,
  • Juan José García-García,
  • Juan José García-García,
  • Juan José García-García,
  • Ruth Aguilar,
  • Iolanda Jordan,
  • Iolanda Jordan,
  • Iolanda Jordan,
  • Gemma Moncunill,
  • Gemma Moncunill

DOI
https://doi.org/10.3389/fimmu.2022.751705
Journal volume & issue
Vol. 13

Abstract

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COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the infectivity dynamics of SARS-CoV-2 in pediatric populations and guide public health interventions, particularly if this population is not fully vaccinated. We evaluated the utility of high-throughput Luminex assays to quantify saliva IgM, IgA and IgG antibodies against five SARS-CoV-2 spike (S) and nucleocapsid (N) antigens in a contacts and infectivity longitudinal study in 122 individuals (52 children and 70 adults). We compared saliva versus serum/plasma samples in infected children and adults diagnosed by weekly RT-PCR over 35 days (n=62), and those who consistently tested negative over the same follow up period (n=60), in the Summer of 2020 in Barcelona, Spain. Saliva antibody levels in SARS-CoV-2 RT-PCR positive individuals were significantly higher than in negative individuals and correlated with those measured in sera/plasmas. Asymptomatic infected individuals had higher levels of anti-S IgG than symptomatic individuals, suggesting a protective anti-disease role for antibodies. Higher anti-S IgG and IgM levels in serum/plasma and saliva, respectively, in infected children compared to infected adults could also be related to stronger clinical immunity in them. Among infected children, males had higher levels of saliva IgG to N and RBD than females. Despite overall correlation, individual clustering analysis suggested that responses that may not be detected in blood could be patent in saliva, and vice versa.In conclusion, measurement of SARS-CoV-2-specific saliva antibodies should be considered as a complementary non-invasive assay to serum/plasma to determine COVID-19 prevalence and transmission in pediatric populations before and after vaccination campaigns.

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