Determination of histamine levels in fresh fish using Near Infrared (NIR) technology

Abstract

Fresh fish and fish products are highly perishable food. Development of fast, secure and non- destructive technique for estimation the presence and quantity of components which are related with safety of food products is of great interest for science but also and for the industry sector. Above-mentioned idea is the aim of this research work, precisely, determination of histamine levels in fresh fish with the use of different near infrared handheld devices. Histamine is one of biogenic amines among putrescine, cadaverine, tyramine which are non-volatile amines produced post mortem and are formed from decarboxylation of specific free amino acids in fish or shellfish tissue. Reason why we choose to developing technique for evaluation of histamine levels is EU Regulation 2073/2005 requires the determination of histamine at three different levels: 100, 200 and 400 mg/kg. For reaching the goal we decided to use paper-based technology for collecting samples for evaluation using Whatman grade 2 qualitative filter papers. In the first phase for validation of NIR devices specifications, and for creation of calibration curve we used histamine solution with 0.5, 1, 2.5 and 5 %. Results showed regression of R2 = 0.69 which indicates that NIR devices can be promising tools for spotting histamine beside small molecular weight.