Glutathione system participation on neutrophils functional status during acute inflammation

Abstract

There was conducted the study of glutathione system status (the content of reduced, oxidized, protein-bound glutathione, SHgroups of proteins and glutathionperoxydase activity) and functional properties (the production of hydroxyl radical, IL-8, TNF-α and myeloperoxidase activity) of neutrophils received from people with community-acquired pneumonia. There was revealed the role of reduced and protein-bound glutathione in the regulation of myeloperoxidase activity and production of hydroxyl radical by blood neutrophils when incubating cells with glutathione synthesis blocker de novo (buthioninesulfoximine). and catalase inhibitor (3-amino-1,2,4-triazole). It was ascertained that glutathione synthesis de novo is more important than catalase activity (inhibition by 3-amino 1,2,4-triasole) to support neutrophil functional condition.

Keywords

• глутатион
• нейтрофилы
• острое воспаление
• интерлейкины