40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) and Tma64 (eIF2D) in 40S subunit recycling

David J. Young; Sezen Meydan; Nicholas R. Guydosh

doi:10.1038/s41467-021-23223-8

Nature Communications (May 2021)

40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) and Tma64 (eIF2D) in 40S subunit recycling

David J. Young,
Sezen Meydan,
Nicholas R. Guydosh

Affiliations

David J. Young: Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health
Sezen Meydan: Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health
Nicholas R. Guydosh: Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health

DOI: https://doi.org/10.1038/s41467-021-23223-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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Following the termination of translation at stop codons, the eukaryotic 60S subunit of the ribosome is removed by the ATPase ABCE1. Here using 40S ribosome footprinting the authors provide a direct demonstration that the yeast orthologs of eIF2D, MCT-1, and DENR recycle the 40S subunits.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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