Polyphenols and Brazilian red propolis incorporated into a total-etching adhesive system help in maintaining bonding durability
Isabel Cristina Celerino de Moraes Porto,
Arthur Bezerra de Barros Rocha,
Iverson Iago Soares Ferreira,
Bruna Muritiba de Barros,
Eryck Canabarra Ávila,
Matheus Corrêa da Silva,
Marcos Paulo Santana de Oliveira,
Teresa de Lisieux Guedes Ferreira Lôbo,
José Marcos dos Santos Oliveira,
Ticiano Gomes do Nascimento,
Jeniffer Mclaine Duarte de Freitas,
Johnnatan Duarte de Freitas
Affiliations
Isabel Cristina Celerino de Moraes Porto
Laboratoty of Characterization and Analysis of Biomaterials, Faculty of Dentistry, Federal University of Alagoas, Campus AC Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57072-970, Maceió, Alagoas, Brazil; Laboratory of Pharmaceutical and Food Analysis, Postgraduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Campus A. C. Simões, CEP 57072-970, Maceió, Alagoas, Brazil; Corresponding author.
Arthur Bezerra de Barros Rocha
Laboratoty of Characterization and Analysis of Biomaterials, Faculty of Dentistry, Federal University of Alagoas, Campus AC Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57072-970, Maceió, Alagoas, Brazil
Iverson Iago Soares Ferreira
Laboratoty of Characterization and Analysis of Biomaterials, Faculty of Dentistry, Federal University of Alagoas, Campus AC Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57072-970, Maceió, Alagoas, Brazil
Bruna Muritiba de Barros
Laboratoty of Characterization and Analysis of Biomaterials, Faculty of Dentistry, Federal University of Alagoas, Campus AC Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57072-970, Maceió, Alagoas, Brazil
Eryck Canabarra Ávila
Laboratoty of Characterization and Analysis of Biomaterials, Faculty of Dentistry, Federal University of Alagoas, Campus AC Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57072-970, Maceió, Alagoas, Brazil
Matheus Corrêa da Silva
Laboratoty of Characterization and Analysis of Biomaterials, Faculty of Dentistry, Federal University of Alagoas, Campus AC Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57072-970, Maceió, Alagoas, Brazil
Marcos Paulo Santana de Oliveira
Laboratoty of Characterization and Analysis of Biomaterials, Faculty of Dentistry, Federal University of Alagoas, Campus AC Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57072-970, Maceió, Alagoas, Brazil
Teresa de Lisieux Guedes Ferreira Lôbo
Laboratory of Pharmaceutical and Food Analysis, Postgraduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Campus A. C. Simões, CEP 57072-970, Maceió, Alagoas, Brazil
José Marcos dos Santos Oliveira
Postgraduate Program in Health Research, Cesmac University Center, Rua Prof. Ângelo Neto, 51, Farol, CEP 57051-530, Maceió, Alagoas, Brazil
Ticiano Gomes do Nascimento
Laboratory of Pharmaceutical and Food Analysis, Postgraduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Campus A. C. Simões, CEP 57072-970, Maceió, Alagoas, Brazil
Jeniffer Mclaine Duarte de Freitas
Laboratory of Pharmaceutical and Food Analysis, Postgraduate Program in Nutrition, Faculty of Nutrition, Federal University of Alagoas, Campus A. C. Simões, CEP 57072-970, Maceió, Alagoas, Brazil
Johnnatan Duarte de Freitas
Department of Chemistry, Federal Institute of Alagoas, Rua Mizael Domingues, 75, Campus Maceió, CEP 57020-600, Maceió, Alagoas, Brazil
Objectives: The aim of this study was to evaluate the degree of conversion and bond strength of a commercial dental adhesive modified by the incorporation of quercetin, resveratrol (RES), and Brazilian red propolis (BRP). Methods: BRP markers were identified using ultra-performance liquid chromatography coupled with a diode array detector, and the antioxidant activity (AAO) of the three substances was analyzed. Single Bond 2 adhesive (3M ESPE) was modified by adding BRP, quercetin, and RES, separately, at 20 μg/mL, 250 μg/mL, and 500 μg/mL, respectively. The degree of conversion (DC) was measured using near-infrared spectroscopy 24 h after photopolymerization. Measurements of the resin-dentin microtensile bond strength (μTBS) were carried out after 1 day and 1 year. Student's t test and ANOVA with Tukey's test were used for data analysis (α = 0.05). Results: The markers daidzein, liquiritigenin, pinobanksin, isoliquiritigenin, formononetin, pinocembrin, and biochanin A were found in the ethanolic extract of BRP. Quercetin, RES, and BRP showed high AAO. The DC of the tested adhesives remained adequate for this category of material, with a slight increase in the DC of adhesives with quercetin and BRP (P > 0.05). Comparisons between μTBS measurements made at 1 day and 1 year showed that, contrary to the control group, μTBS values for all modified adhesives were maintained after 1 year in distilled water (P > 0.05). Conclusions: These findings suggest that quercetin, RES, or BRP might be useful in adhesive dentistry to help improve hybrid layer resistance. Clinical significance: Dentin bonding agents with quercetin, RES, and BRP have potential to increase the longevity of composite restorations.
