Differentiation of human pluripotent stem cells into pancreatic duct-like organoids

Abstract

Summary: The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive.For complete details on the use and execution of this protocol, please refer to Breunig et al. (2021).

Keywords

• Cell culture
• Developmental biology
• Stem Cells
• Cell Differentiation
• Organoids