Crop Breeding and Applied Biotechnology (Jan 2006)
Molecular analysis of the seedlessness character in grape using RAPD markers
Abstract
Forty-eight polymorphic RAPD primers were used to study the genetic similarity of eight grapevine (Vitis spp.)accessions: Itália, Seyve Villard 12327, Seyve Villard 12375, Crimson Seedless, CG 87746, A 1976, Gota de Ouro andCNPUV 154-27. Gota de Ouro, the only Vitis labrusca cultivar, was the most distant cultivar from the others. The populationCNPUV 154 (Seyve Villard 12327 x CG 87746) was evaluated for fresh and dry seed weight. Four phenotypic classes wereconsidered for dry matter percentage (DM%): 1 (DM > 55), 2 (45 < DM < 55), 3 (35 < DM < 45) and 4 (DM < 35). Theobserved frequencies for the classes 1, 2, 3, and 4 were 37, 27, 53, and 9 individuals, respectively. Of the 500 RAPD screenedprimers, 111 were selected as polymorphic. According to BSA, markers generated by OPK02, OPM16, OPS03, and OPU13primers could be related to the region that controls seedlessness.