Nature Communications (Sep 2019)
High levels of AAV vector integration into CRISPR-induced DNA breaks
- Killian S. Hanlon,
- Benjamin P. Kleinstiver,
- Sara P. Garcia,
- Mikołaj P. Zaborowski,
- Adrienn Volak,
- Stefan E. Spirig,
- Alissa Muller,
- Alexander A. Sousa,
- Shengdar Q. Tsai,
- Niclas E. Bengtsson,
- Camilla Lööv,
- Martin Ingelsson,
- Jeffrey S. Chamberlain,
- David P. Corey,
- Martin J. Aryee,
- J. Keith Joung,
- Xandra O. Breakefield,
- Casey A. Maguire,
- Bence György
Affiliations
- Killian S. Hanlon
- Department of Neurobiology, Harvard Medical School
- Benjamin P. Kleinstiver
- Center for Genomic Medicine, Massachusetts General Hospital
- Sara P. Garcia
- Department of Pathology, Harvard Medical School
- Mikołaj P. Zaborowski
- Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital
- Adrienn Volak
- Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital
- Stefan E. Spirig
- Institute of Molecular and Clinical Ophthalmology Basel
- Alissa Muller
- Institute of Molecular and Clinical Ophthalmology Basel
- Alexander A. Sousa
- Molecular Pathology Unit, Massachusetts General Hospital
- Shengdar Q. Tsai
- Department of Hematology, St. Jude Children’s Research Hospital
- Niclas E. Bengtsson
- Department of Neurology, University of Washington
- Camilla Lööv
- Uppsala University, Department of Public Health and Caring Sciences, Geriatrics
- Martin Ingelsson
- Uppsala University, Department of Public Health and Caring Sciences, Geriatrics
- Jeffrey S. Chamberlain
- Department of Neurology, University of Washington
- David P. Corey
- Department of Neurobiology, Harvard Medical School
- Martin J. Aryee
- Department of Pathology, Harvard Medical School
- J. Keith Joung
- Department of Pathology, Harvard Medical School
- Xandra O. Breakefield
- Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital
- Casey A. Maguire
- Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital
- Bence György
- Department of Neurobiology, Harvard Medical School
- DOI
- https://doi.org/10.1038/s41467-019-12449-2
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 11
Abstract
In-depth characterization of adeno-associated virus (AAV)-mediated CRISPR delivery is still lacking. Here, the authors show high levels of integration into Cas9-induced double-strand breaks (DSBs) in therapeutically relevant genes in vivo.
