Development of biocompatible Ni-ferrite nanoparticles with PEG-coated for magnetic hyperthermia
K. Ohara,
T. Moriwaki,
K. Nakazawa,
T. Sakamoto,
K. Nii,
M. Abe,
Y. Ichiyanagi
Affiliations
K. Ohara
Department of Physics, Graduate School of Engineering Science, Yokohama National University, Yokohama, Kanagawa 240-8501, Japan
T. Moriwaki
Department of National Environment, Graduate School of Environment and Information Science, Yokohama National University, Yokohama, Kanagawa 240-8501, Japan
K. Nakazawa
Department of National Environment, Graduate School of Environment and Information Science, Yokohama National University, Yokohama, Kanagawa 240-8501, Japan
T. Sakamoto
Department of Physics, Graduate School of Engineering Science, Yokohama National University, Yokohama, Kanagawa 240-8501, Japan
K. Nii
Department of Physics, Graduate School of Engineering Science, Yokohama National University, Yokohama, Kanagawa 240-8501, Japan
M. Abe
Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
Y. Ichiyanagi
Department of Physics, Graduate School of Engineering Science, Yokohama National University, Yokohama, Kanagawa 240-8501, Japan
In this study, NiFe2O4 nanoparticles were prepared as a thermophilic agent for magnetic thermotherapy. To improve biocompatibility, the particles were coated with polyethylene glycol, which is hydrophilic and nontoxic; X-ray diffraction measurements confirmed that the particle size could be controlled to 3, 6, 9, and 17 nm. Fourier-transform infrared spectroscopy confirmed PEG modification to the particles. Biocompatibility with cells were improved by coating with PEG of high molecular weight. The heat-generating effect of the particles was investigated by measuring the magnetic susceptibility and temperature rise in an AC magnetic field. Furthermore, a device using a zero-voltage switching circuit was developed to generate heat. Finally, in vitro experiments using human breast cancer cells were successfully performed to confirm the onset of the hyperthermia effect.
