Changes in Serum Proteins in Cats with Obesity: A Proteomic Approach
Esmeralda Cañadas-Vidal,
Alberto Muñoz-Prieto,
Dina Rešetar Maslov,
Ivana Rubić,
Juan C. González-Sánchez,
Juan D. Garcia-Martinez,
José J. Ceron,
Vladimir Mrljak,
Luis Pardo-Marin,
Silvia Martinez-Subiela,
Asta Tvarijonaviciute
Affiliations
Esmeralda Cañadas-Vidal
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain
Alberto Muñoz-Prieto
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain
Dina Rešetar Maslov
Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
Ivana Rubić
Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
Juan C. González-Sánchez
BioQuant, Heidelberg University, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany
Juan D. Garcia-Martinez
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, Calle Campus Universitario, Espinardo, 30100 Murcia, Spain
José J. Ceron
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain
Vladimir Mrljak
Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
Luis Pardo-Marin
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain
Silvia Martinez-Subiela
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain
Asta Tvarijonaviciute
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain
Obesity is defined as the excessive accumulation of adipose tissue and is currently the most common disease in cats. Similarly to humans, obesity negatively impacts the health and welfare of cats, predisposing them to many other disorders. The objective of this study was to compare the serum proteomes of normal-weight and overweight/obese cats, aiming to gain insights into the physiopathology of feline obesity and potentially identify new biomarkers. For this, serum samples from a total of 20 adult neutered domestic shorthair client-owned cats, ten normal weight and ten overweight/obese, were submitted to tandem mass tags labelling and liquid chromatography-mass spectrometry (LC-MS/MS) analysis. A total of 288 proteins were detected in the serum samples. Out of these, 12 proteins showed statistically significant differences in abundance between control cats and cats with obesity, namely Ig-like domain-containing protein, Alpha-2-HS-glycoprotein, Complement C8 gamma chain, An-tithrombin-III, Serpin family A member 1, Complement factor H, C3-beta-c, Albumin, C4b-binding protein alpha chain, Alpha-1-B glycoprotein, Solute carrier family 12 member 4, and Fibronectin. Overall this report identifies new proteins involved and provides additional knowledge about the physiopathological changes related to feline obesity.
